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<DIV><FONT face=Arial size=2>Dear colleauges,</FONT></DIV>
<DIV><FONT face=Arial><STRONG>Mainly for those who are
interested in membrane proteins.</STRONG></FONT></DIV>
<DIV><FONT face=Arial size=2> For the determination of of
the molecular masses of membrane proteins (MP) are used
different methodes,</FONT></DIV>
<DIV><FONT face=Arial size=2>some are complicated to run
or /and to calculate the the protein-only of the
detergent complex. Since long time </FONT></DIV>
<DIV><FONT face=Arial size=2>I deal with this probleme and in the
year 2000 we published in BBA N°1464
page199-206</FONT></DIV>
<DIV><FONT face=Arial size=2>>>Molecular weight determination of MP
by SE at sucrose or Nycodenz-adjusted density of hydated
det.micelle.<<</FONT></DIV>
<DIV><FONT face=Arial size=2> by Ariel Lustig, Andreas Engel,
Georgios Tsiotis, Ehud M Landau ,Werner Baschong</FONT></DIV>
<DIV><FONT face=Arial size=2>Some other groups who tried different methodes
criticized (partly) and it is their right . This
practical proceedure</FONT></DIV>
<DIV><FONT face=Arial size=2>that is described in detail does not obey
the the chapter "Detergent-solubilized Protein" of the Beckman
booklet of </FONT></DIV>
<DIV><FONT face=Arial size=2>Greg Ralstone ! even so...we can live
with it.</FONT></DIV>
<DIV><FONT face=Arial size=2>As shortly appeared the 5<FONT
size=1>th </FONT><FONT size=2>publication using this methode in
quite good papers, the methode seems not to be
</FONT></FONT></DIV>
<DIV><FONT face=Arial size=2>the worst and is applicable. I like to
give you the list:</FONT></DIV>
<DIV><FONT face=Arial size=2> The J. of Biological Chemestry
vol 276 N° 33 Aug.2001 p.30845</FONT></DIV>
<DIV><FONT face=Arial size=2>>>Sarcolipin , the shorter Homolouge
of Phospholamban...<<..by Simon Hellstern, Stefano Pegorarog,
C B Karim, A Lustig, D Thomas L moroder. Juergen
Engel. In this paper even 2 detergents were emploid
and concentration dependence could be recorded</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2> The Hoffman La Roche Pharma
Research published in (BBRC) Bioche. and Bioph. Research
Commun.315</FONT></DIV>
<DIV><FONT face=Arial size=2>2004 p247 >> the monotopic
membrane protein human oxidosqualene cyclase....>></FONT></DIV>
<DIV><FONT face=Arial size=2>by Armin Ruf, Francis Mueller ,B Arcy
...and ..R Thoma</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>In several different detergents the protein
run by SE and it is easy to see who are
the best and in which concentration and density worth
to use. The OG was matched with sucrose and
hydration corrected.</FONT></DIV>
<DIV><FONT face=Arial size=2>Interesting to learn how some
MP " feel well" in a certain Detergent and
other not as shown With </FONT></DIV>
<DIV><FONT face=Arial size=2>MAO B or MAO A Here the best
Chois was ZW Zwittergent 3-12 ro =10449 and made
gravitational transparent </FONT></DIV>
<DIV><FONT face=Arial size=2>with buffer^/D<FONT size=1>2</FONT>O
</FONT></DIV>
<DIV><FONT face=Arial size=2>PNAS vol 102 N° 36 sept 2005
p12684...</FONT></DIV>
<DIV><FONT face=Arial size=2>>> Three -dimensional structure of
human monoamine oxidase A MAO A ...MAO B</FONT></DIV>
<DIV><FONT face=Arial size=2>Luigi de Colibus, Min Li , Cl. Binda, A
Lustig, D. Edmondson A. Mattevi</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>The Aquaporin not as several
Porins who forms a three-mer channel could recently be
determined in</FONT></DIV>
<DIV><FONT face=Arial size=2>J Mol Biol Communication November 2005
is tetrameric.</FONT></DIV>
<DIV><FONT face=Arial size=2>>>Co-axial association of Recombinant
EYE LENS Aquaporin.....<<</FONT></DIV>
<DIV><FONT face=Arial size=2> D. v. Palanivelu , D Kozno, And. Engel.
K. suda, a. Lustig P.Agre, T.Schirmer.</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>The contribution of the anal.
ultracentrifugation is mainy to show the behavior
of Mem-protein in solution,</FONT></DIV>
<DIV><FONT face=Arial size=2>since in SDS they run wrong so also in sefadex or
similar cloumns. In EM detergent obscurs etc..</FONT></DIV>
<DIV><FONT face=Arial size=2>L-Scatteringgive only averages.</FONT></DIV>
<DIV><FONT face=Arial size=2>The sucrose for matching
the detergent micelles causes to strong hydration of
the detergent-micell. Incase of </FONT></DIV>
<DIV><FONT face=Arial size=2>DM from ro 1.22 to 1.12.</FONT></DIV>
<DIV><FONT face=Arial size=2>and the protein (as longtime was
known with high molar salt that the shift may be from ro
1.35 to 1.28 about) so the hydration as we corrected it is not so
precise but helps for the contribution of the shown
papers.</FONT></DIV>
<DIV><FONT face=Arial size=2>yours ,,,, ariel</FONT></DIV>
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