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<DIV><FONT face=Arial size=2>Dear Yu-Chia Cheng, dear colleauges,</FONT></DIV>
<DIV><FONT face=Arial size=2>working at <STRONG>2 molar</STRONG> am.sulfate
you can't first at all expect to achieve the same molecular masses as in
</FONT></DIV>
<DIV><FONT face=Arial size=2>normal buffers (low molarity) due
to the hydration effect that causes to use a
different Vbar as </FONT></DIV>
<DIV><FONT face=Arial size=2>published or calculated from A.-acids. This has
been known since the 50-60ers of the last
century.</FONT></DIV>
<DIV><FONT face=Arial size=2> S -vel. can't help you either, since
the shape and so the friction may be different as in normal
buffer, </FONT></DIV>
<DIV><FONT face=Arial size=2>but this fact can be learned
if you make a control of each of the two
samples , I mean you ran in a separate cell the oval. , the
lyso. and the mixture . A big help could be also to do
so with SE.</FONT></DIV>
<DIV><FONT face=Arial size=2>Let us think what we may
expect : 1) an hetrogene mixed specie from lys. to oval. 2) a
very nice homogene specie if the have bound that gives an
average value , that is smaller then the oval. 3) an
homogene specie if the have bound , that gives a value of both
together. 4) is similar like "3" but if occurs an </FONT></DIV>
<DIV><FONT face=Arial size=2>association multipplied values of " 3" you
may find.</FONT></DIV>
<DIV><FONT face=Arial size=2>I think that with many programs
you may analyse Ln C versus r<FONT size=1> squ. </FONT><FONT
size=2>that should give a straight line</FONT></FONT></DIV>
<DIV><FONT face=Arial size=2>if it is as "2" or "3" and if it is "1"your
line cann't be straight !!!</FONT></DIV>
<DIV><FONT face=Arial size=2>We use >>Segal <<as many of
you also tried it and found it easy to use. The address is now
shorter changed and shorter: <A
href="http://www.biozentrum.unibas.ch/auc">www.biozentrum.unibas.ch/auc</A> </FONT></DIV>
<DIV><FONT face=Arial size=2>yours
ariel .</FONT></DIV></BODY></HTML>