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<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Allen,<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>I had a similar experience two years ago with a cell that
had an Al filled centerpiece in it. The centrifuge was running at 55000rpm and
we were collected scans at one minute intervals. After about 70 minutes, the one
sector emptied between consecutive scans breaking the centerpiece and one
of the sapphire windows. Prior to that scan, the meniscus, which
nearly the same for both sectors, had not varied for either sector. I
have always presumed that one of the windows cracked and allowed the sample to
escape. So, I think my message is that no matter how careful you are bad (and
expensive) things can happen with high g loads.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>John<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>John Burgner<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Director, Biophysics Core<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>The <u1:place u2:st="on"><u1:PlaceName u2:st="on"><st1:place
w:st="on"><st1:PlaceName w:st="on">Bindley</u1:PlaceName></st1:PlaceName> <u1:PlaceName u2:st="on"><st1:PlaceName
w:st="on">Bioscience</u1:PlaceName></st1:PlaceName> <u1:PlaceType u2:st="on"><st1:PlaceType
w:st="on">Center</u1:PlaceType></u1:place></st1:PlaceType></st1:place><o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Dept of Biological Sciences<o:p></o:p></span></font></p>
<p class=MsoNormal><u1:place u2:st="on"><u1:PlaceName u2:st="on"><st1:place
w:st="on"><st1:PlaceName w:st="on"><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>Purdue</span></font></st1:PlaceName><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'></u1:PlaceName>
<u1:PlaceType u2:st="on"><st1:PlaceType w:st="on">University</u1:PlaceType></st1:PlaceType></span></font></st1:place></u1:place><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'><o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>765-494-4960</span></font><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'><o:p></o:p></span></font></p>
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12.0pt'><o:p> </o:p></span></font></p>
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