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<DIV><FONT face=Arial size=2>Dear Richard,dear Christine and colleauges
who are interested in the liposome subject,</FONT></DIV>
<DIV><FONT face=Arial size=2>Richard, you gave me the hint :DENSITY! and
reminded me that I tryed to run not only an rapid dynamical
gradient (A. Lustig et al BBA1115 (1991 )89 -95 BBAGEN 23614 that is
<STRONG>not</STRONG> suitable for this density range (ro 1.0
to 1.01) later I discribed at the X<FONT size=1>th </FONT><FONT
size=2>Symposium 1997 in Regensburg p.26 a static Nycodenz
</FONT></FONT></DIV>
<DIV><FONT face=Arial size=2>density gradient that I
calibrated with tar 1.022and poly-sterat 1.007 particles.
also in my gradient </FONT></DIV>
<DIV><FONT face=Arial size=2>liposomes do not show a unic
gradient(several bands that could not be repeated from preparation
to preparation). As you also I think they are dispersed
but do not float?. Both methode we used to determine particles at
about ro 1.1to1.2 of lipid/protein.</FONT></DIV>
<DIV><FONT face=Arial size=2> Actually an even a better
methode is the one of Ching-Hsien Huang and James P Charlton JBC vol 216
N°8 April 1971"</FONT></DIV>
<DIV><FONT face=Arial size=2>Determination of </FONT></DIV>
<DIV><FONT face=Arial size=2>Part. Spec.Vol. by sed. vel. methode".</FONT></DIV>
<DIV><FONT face=Arial size=2>Like you if the particles are not mono, I'm
as we say in german <am ende unser Lateins<</FONT></DIV>
<DIV><FONT face=Arial size=2>regards...ariel</FONT></DIV></BODY></HTML>