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<DIV><SPAN class=502370221-04052005><FONT size=2>Sorry Jack, but I have to
disagree with you. First, you are talking about velocity analysis, not
equilibrium. The Beckman software is by far the most user-friendly equilibrium
analysis package I have seen. Its algorithms are equivalent to those
in NONLIN, but you don't have to fight with obscure speed factors etc., and
it actually gives you answers in real molecular weight (at least when it works
correctly), which NONLIN never does. Yes, there are more sophisticated
things that can be done, but if you don't need them, why struggle?
</FONT></SPAN></DIV>
<DIV><SPAN class=502370221-04052005><FONT size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=502370221-04052005><FONT size=2>Second, what is wrong with
simple? Isn't it particularly important for new users to achieve some quick
success so they are encouraged to do more, including learning these 'powerful
and elegant' approaches?</FONT></SPAN></DIV>
<DIV><SPAN class=502370221-04052005><FONT size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=502370221-04052005><FONT size=2>John</FONT></SPAN></DIV></DIV>
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<DIV class=OutlookMessageHeader lang=en-us dir=ltr align=left><FONT
face=Tahoma size=2>-----Original Message-----<BR><B>From:</B>
rasmb-admin@server1.bbri.org [mailto:rasmb-admin@server1.bbri.org] <B>On
Behalf Of </B>John Correia<BR><B>Sent:</B> Wednesday, May 04, 2005 11:34
AM<BR><B>To:</B> rasmb@server1.bbri.org; apl3@york.ac.uk<BR><B>Cc:</B>
John.McGeehan@port.ac.uk<BR><B>Subject:</B> Re: [RASMB] Origin software and
vbar<BR><BR></FONT></DIV>
<DIV><FONT size=2>This may seem to be an odd question, but why are you using
the Beckman Origin software? Depending upon your guru (Schuck &
sedfit or Stafford & SEDANAL or Demeler & Ultrascan
or ... ) there are incredible packages being developed out there -
& they all work when applied to appropriate systems - we use a combination
of c(s) g(s) to establish a hypothesis, maybe some weight average fitting
to explore energetics, and then global direct boundary fitting to
hetero models with SEDANAL as the final step! I realize Beckman is
trying to make it uniform & simple, but with the versatility, power and
elegance of these new analysis methods, why would you settle for
simple?</FONT></DIV>
<DIV> </DIV>
<DIV><FONT
size=2>-------------------------------------------------------------------<BR> Dr.
John J. "Jack" Correia<BR> Department of Biochemistry<BR> University
of Mississippi Medical Center<BR> 2500 North State
Street<BR> Jackson, MS 39216<BR> (601)
984-1522
<BR> fax (601)
984-1501
<BR> email address: <A
href="mailto:jcorreia@biochem.umsmed.edu">jcorreia@biochem.umsmed.edu</A>
<BR> homepage location: <A
href="http://biochemistry.umc.edu/correia.html">http://biochemistry.umc.edu/correia.html</A><BR> dept
homepage location: <A
href="http://biochemistry.umc.edu/">http://biochemistry.umc.edu/</A><BR>-------------------------------------------------------------------<BR> <BR> <BR></FONT></DIV></BLOCKQUOTE></BODY></HTML>