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<title>Elevated temperature operation with a turbomoledcular pump</title>
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<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Dear Walter and RASMB-ers<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>We are definitely interested in implementing this
improvement.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Regards<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Amanda<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>___________________________________________________<o:p></o:p></span></font></p>
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5.0pt;margin-left:0in'><font size=2 face="Comic Sans MS"><span
style='font-size:10.0pt;font-family:"Comic Sans MS"'>Amanda Nourse (PhD)<o:p></o:p></span></font></p>
<p class=MsoNormal style='mso-margin-top-alt:5.0pt;margin-right:0in;margin-bottom:
5.0pt;margin-left:0in'><font size=2 face="Comic Sans MS"><span
style='font-size:10.0pt;font-family:"Comic Sans MS"'>Section Leader: Molecular
Interactions Analysis<o:p></o:p></span></font></p>
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<st1:PlaceType w:st="on">Center</st1:PlaceType></span></font></st1:place><font
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for Bioinformatics and Biotechnology<o:p></o:p></span></font></p>
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<st1:State w:st="on">TN</st1:State> <st1:PostalCode w:st="on">38105</st1:PostalCode>
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style='font-size:10.0pt;font-family:"Comic Sans MS"'>901-495-4845 (W)<o:p></o:p></span></font></p>
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style='font-size:10.0pt;font-family:"Comic Sans MS"'>901-495-2945 (Fax)<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
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<p class=MsoNormal><b><font size=2 face=Tahoma><span style='font-size:10.0pt;
font-family:Tahoma;font-weight:bold'>From:</span></font></b><font size=2
face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma'>
rasmb-admin@server1.bbri.org [mailto:rasmb-admin@server1.bbri.org] <b><span
style='font-weight:bold'>On Behalf Of </span></b>Walter Stafford<br>
<b><span style='font-weight:bold'>Sent:</span></b> Friday, March 11, 2005 11:18
AM<br>
<b><span style='font-weight:bold'>To:</span></b> rasmb@server1.bbri.org<br>
<b><span style='font-weight:bold'>Subject:</span></b> [RASMB] Elevated
temperature operation with a turbomoledcular pump.</span></font><o:p></o:p></p>
</div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>Dear RASMB-ers<o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
</div>
<div><x-tab>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'> </x-tab>I have been testing
a turbomolecular pump as a replacement for the standard diffusion pump on the
XL-I. I am writing to report the results of that experiment.<o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>On my machine, with the standard diffusion pump installed, I have not
been able to run above about 23 degrees without having serious fogging of the
interference condensing lens after about 15 minutes, making it impossible to
take data.<o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>About a month ago, with the help of the Beckman Serviceman and folks
from the Axiden Division of Alcatel Vacuum Technology</span></font><font
size=1 color=black face="Lucida Grande"><span style='font-size:7.5pt;
font-family:"Lucida Grande";color:black'> {http://www.adixen-usa.com/</span></font>},
we installed an ATP80 TMP in place of the diffusion pump. Other than a flange
that had to be machined to mate the TMP to the diffusion pump flange, it was a
direct replacement. The original rough pump was used to back up the TMP. <font
size=1 color=black face="Lucida Grande"><span style='font-size:7.5pt;
font-family:"Lucida Grande";color:black'> see:
{http://www.adixen.com/all/dyn/products/products.php?id_prod=20&lg=us</span></font>}<o:p></o:p></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>So far the results have been quite remarkable. After the first test of
running for 4 days at 40 degrees, the optics were clean. The chamber was
actually cleaner after the run than before. The TMP seemed to have removed all
the residual oil that we were unable to remove after replacing the diffusion
pump. It pumps down to 3-5 micons in less than 5 minutes and ultimately to
about 1-2 microns. The fringe patterns seem to be more stable (not sure exactly
why, but I was informed that the regular diffusion pump heater normally cycles
on and off; the flexing associated with that may explain it).<o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>We use it routinely now for all runs and couldn't be happier with it.<o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>Several labs have expressed an interest in installing one on their
machines.<o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>However, Beckman will not support the pump or provide one as standard
equipment unless there is sufficient interest. A proper, Murphy-proof
installation would require a firmware change and field service support to allow
it to be included under the service contract and warranty.<o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>I would like to take a survey of those who might be interested, so that
we can convey that expression of interest to Beckman.<o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>If you have an interest and think that running at higher than 20
degrees or higher precision might be useful to you, please respond to this
email by replying to the RASMB. I'll collect the responses and comments and
forward them to the folks at Beckman.<o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>It has opened new vistas for our research.<o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>Thanks<o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>Walter<o:p></o:p></span></font></p>
</div>
<pre><font size=2 face="Courier New"><span style='font-size:10.0pt'><x-sigsep>-- <o:p></o:p></span></font></pre>
<div>
<p class=MsoNormal><font size=3 color="#0b0963" face="Times New Roman"><span
style='font-size:12.0pt;color:#0B0963'></x-sigsep>########################################################################</span></font><o:p></o:p></p>
</div>
<div>
<p class=MsoNormal><font size=3 color="#0b0963" face="Times New Roman"><span
style='font-size:12.0pt;color:#0B0963'>Walter F. Stafford III, Ph.D.</span></font><o:p></o:p></p>
</div>
<div>
<p class=MsoNormal><font size=3 color="#0b0963" face="Times New Roman"><span
style='font-size:12.0pt;color:#0B0963'>Analytical Ultracentrifugation Research
Laboratory<br>
Senior Scientist<br>
<st1:City w:st="on"><st1:place w:st="on">Boston</st1:place></st1:City>
Biomedical Research Institute<br>
<st1:Street w:st="on"><st1:address w:st="on">64 Grove Street</st1:address></st1:Street><br>
<st1:place w:st="on"><st1:City w:st="on">Watertown</st1:City>, <st1:State
w:st="on">MA</st1:State> <st1:PostalCode w:st="on">02472</st1:PostalCode></st1:place>-2829<br>
<br>
main:(617) 926-8040<br>
tel: (617) 658-7808<br>
fax: (617) 972-1753<br>
<br>
mailto:stafford@bbri.org</span></font><o:p></o:p></p>
</div>
<div>
<p class=MsoNormal><font size=3 color="#0b0963" face="Times New Roman"><span
style='font-size:12.0pt;color:#0B0963'>http://www.bbri.org/faculty/stafford/Stafford.html</span></font><o:p></o:p></p>
</div>
<div>
<p class=MsoNormal><font size=3 color="#0b0963" face="Times New Roman"><span
style='font-size:12.0pt;color:#0B0963'>#################################################################</span></font><o:p></o:p></p>
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