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<DIV><FONT size=2>I have done experiments on microtubule samples in excess of
5000 s at 1-3 K - a combination of C(s) and WD-DCDT work well for
analysis.</FONT></DIV>
<DIV><FONT size=2></FONT> </DIV>
<DIV><FONT size=2>I did notice that my XLA did not seem to like calibrating at
1000 rpm and I had to calibrate at 3 K and then slow the speed to 1 K to
maximize the number of scans. This may not be a unuversal problem.
It was also helpful to only run one sample at a time or to scan a narrow region
of the cell to maximixe data acquisition (& thus using WD-DCDT
analysis).</FONT></DIV>
<DIV><FONT size=2></FONT> </DIV>
<DIV><FONT size=2>It is worth noting vanHolde & Baldwin (1958) measured the
s value of sucrose & Yphantis measured the MW of air (unpublished) by
sealing only one sector of a double sector cell & collecting interference
data. For A + B system the issue may be more the fractional change in
MW than the absolute change.</FONT></DIV>
<DIV><FONT size=2></FONT> </DIV>
<DIV><FONT size=2></FONT> </DIV>
<DIV> </DIV>
<DIV> </DIV>
<DIV>-------------------------------------------------------------------<BR> Dr.
John J. "Jack" Correia<BR> Department of Biochemistry<BR> University
of Mississippi Medical Center<BR> 2500 North State Street<BR> Jackson,
MS 39216<BR> (601)
984-1522
<BR> fax (601)
984-1501
<BR> email address: <A
href="mailto:jcorreia@biochem.umsmed.edu">jcorreia@biochem.umsmed.edu</A>
<BR> homepage location: <A
href="http://biochemistry.umc.edu/correia.html">http://biochemistry.umc.edu/correia.html</A><BR> dept
homepage location: <A
href="http://biochemistry.umc.edu/">http://biochemistry.umc.edu/</A><BR>-------------------------------------------------------------------<BR> <BR> <BR><BR><BR>>>>
"Qin Zou" <heat-capacity@indy.rr.com> 01/25/05 08:31PM
>>><BR></DIV>
<DIV class=Section1>
<P class=MsoNormal><FONT face=Arial size=2><SPAN
style="FONT-SIZE: 10pt; FONT-FAMILY: Arial">Dear all,<?xml:namespace prefix = o
ns = "urn:schemas-microsoft-com:office:office" /><o:p></o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT face=Arial size=2><SPAN
style="FONT-SIZE: 10pt; FONT-FAMILY: Arial">What is the maximum <SPAN
class=SpellE>smax</SPAN> that can be used in c(s) <SPAN class=GramE>analysis
?</SPAN> I have tried 614s but the program crashed if <SPAN
class=SpellE>smax</SPAN> is larger than that. So if one wants to look at fairly
large particle like John Philo did with adenovirus, <SPAN
class=SpellE>sedfit</SPAN> is not the good choice? Also, what would be the good
rotor speed if one wants to look at the particle size of 5-7MDa? <SPAN
style="mso-spacerun: yes"> </SPAN>John Philo used 1200-3000 rpm for 150MDa.
Thanks for any input.<o:p></o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT face=Arial size=2><SPAN
style="FONT-SIZE: 10pt; FONT-FAMILY: Arial"><o:p> </o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT face=Arial size=2><SPAN
style="FONT-SIZE: 10pt; FONT-FAMILY: Arial">Chin <SPAN
class=SpellE>Zou</SPAN><o:p></o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT face=Arial size=2><SPAN
style="FONT-SIZE: 10pt; FONT-FAMILY: Arial">Eli Lilly and
Company<o:p></o:p></SPAN></FONT></P></DIV></BODY></HTML>