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<DIV><FONT face=Arial size=2>Dear Holger, dear colleauges,</FONT></DIV>
<DIV><FONT face=Arial size=2>I believe that your main interest is to achieve the
Mw of a membrane -prot compex MPC,were ATP has to be </FONT></DIV>
<DIV><FONT face=Arial size=2>included . In such case for not MPC I
shifted , if The ATP concentration is not too high to 284 -288
nm, there</FONT></DIV>
<DIV><FONT face=Arial size=2>the influence is minimal.</FONT></DIV>
<DIV><FONT face=Arial size=2>using theXLA. I have no idea about your
mentioned detergent micelle density, but if it is above 1.08 so you cann't
use D<FONT size=1>2</FONT>O as densifier for gravitational transparency. I like
to mention my(at al) BBA paper 1464 (2000) 199-206</FONT></DIV>
<DIV><FONT face=Arial size=2>and also the one of Armin Ruf et al BBRC
315(2004) 247-254.</FONT></DIV>
<DIV><FONT face=Arial size=2>Using sucrose as densifier very well matched
with the reference channel,(same filling hight but not too long filling
hight and moderated speedsso that the detergent will remain sure over
CMC) interference is possible. I dont believe that dialysing is
possible at least it concerns many MP and detergents.</FONT></DIV>
<DIV><FONT face=Arial size=2>SV was used only as a controll-instrument if
a single boundary can be achieved, but the S-observed has no
relation to any to MW or whatever where the viscosity hard to mesure. so S<FONT
size=1>20W </FONT>has also no meaning.</FONT></DIV>
<DIV><FONT face=Arial size=2>I hope to give you soon another paper adress
that is now in revision.</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>Greeeting to all </FONT></DIV>
<DIV><FONT face=Arial size=2> ariel Attention of my new e-mail
address</FONT></DIV>
<DIV><FONT face=Arial size=2> (I'm now starting my activity as
private person with consulting also at my old place</FONT></DIV>
<DIV><FONT face=Arial size=2>the Biozentrum in Basel)</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV><PRE> </PRE>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=1></FONT> </DIV><PRE><STRONG></STRONG> </PRE>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>but if I just like to remind
</FONT></DIV></BODY></HTML>