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<div><font face="Arial" color="#000000">Dear Colleagues,</font></div>
<div><font face="Arial" color="#000000"><br></font></div>
<div><font face="Arial" color="#000000">A postdoctoral position is
available to study the DNA- and protein-interactions of the human
O6-alkylguanine-DNA alkyltransferase protein. This protein plays
an important role in the repair of alkylated DNA and is also a target
of chemotherapeutic agents. The current project includes
preparation of recombinant proteins, characterization of their binding
to target and non-specific DNAs and analysis of their protein-protein
interactions, using biochemical and biophysical techniques.
Some examples of our work in this area are:</font></div>
<div><font face="Arial" color="#000000"><br></font></div>
<div><font face="Arial" color="#000000">DNA Binding Mechanisms of
O6-Alkylguanine-DNA Alkyltransferase: Stoichiometry and Effects of DNA
Base Composition and Secondary Structures on Complex Stability.<b>
M.G. Fried</b>, S. Kanugula, J.L. Bromberg and A.E. Pegg,<i>
Biochemistry</i><u> 35</u>, 15295-15301 (1996).</font></div>
<div><font face="Arial" color="#000000"><br></font></div>
<div><font face="Arial" color="#000000">Effects of Zinc Occupancy on
Human O6 -Alkylguanine-DNA Alkyltransferase J.J. Rasimas, S.
Kanugula, P.M. Dalessio, I.J. Ropson,<b> M.G. Fried</b> and A.E.
Pegg.<i> Biochemistry</i><u> 42</u>, 980-990 (2003).</font></div>
<div><font face="Arial" color="#000000"><br></font></div>
<div><font face="Arial" color="#000000">Interaction of O6-Alkylguanine
DNA Alkyltransferase with DNA: Effects of Protein- and
DNA-Alkylation. J. J. Rasimas, A. E. Pegg and<b> M.G.
Fried</b>.<i> J. Biol. Chem.</i><u> 278</u>, 7973-7980
(2003).</font></div>
<div><font face="Arial" color="#000000"><br></font></div>
<div><font face="Arial" color="#000000">Active-Site Alkylation
Destabilizes Human<i> O</i>6-Alkylguanine DNA Alkyltransferase. J.J.
Rasimas, P. Dalessio, I. Ropson, A.E. Pegg and<b> M.G. Fried</b>.<i>
Protein Science</i>,<u> 13</u>, 301-305 (2004).</font></div>
<div><font face="Arial" color="#000000"><br></font></div>
<div><font face="Arial" color="#000000">Individuals who wish to apply
for this position should send a letter stating their interests and
qualifications, a curriculum vitae, and the names and addresses
(email, postal and telephone number) of three references to me at the
address given below.</font></div>
<div><font face="Arial" color="#000000"><br>
</font><font face="Arial">Thanks very much!</font></div>
<div><font face="Arial">Sincerely,</font></div>
<div><font face="Arial"><br></font></div>
<div><font face="Arial">Mike Fried</font></div>
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</pre></x-sigsep>
<div><font color="#000000">Michael G. Fried, Ph.D.<br>
Professor <span
></span
> <span
></span
> <span
></span
> <span
></span
> <span
></span> <br>
Department of Biochemistry and Molecular Biology,
H171 <br>
The Pennsylvania State University College of
Medicine <br
>
500 University Drive<br>
Hershey, PA 17033<br>
<br>
email: mfried@psu.edu<br>
Telephone: (717) 531-5250<br>
FAX: (717) 531-7072</font><br>
<font color="#000000"></font></div>
<div><font
color="#000000"
>http://fred.hmc.psu.edu/ds/retrieve/fred/investigator/mfried</font></div
>
<div><font color="#000000"><br></font></div>
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