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<DIV><SPAN class=868041617-12032004><FONT face=Arial color=#0000ff
size=2>Samantha and all,</FONT></SPAN></DIV>
<DIV><SPAN class=868041617-12032004><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=868041617-12032004><FONT face=Arial color=#0000ff size=2>The
answer to the many "why isn't X compound in the SEDNTERP
buffer database" questions that have gone out over RASMB over the years is
always the same: all of the data we have are already in there. If compound X is
not there, it means that no one has any data (or at least not that they have
released in public).</FONT></SPAN></DIV>
<DIV><SPAN class=868041617-12032004><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=868041617-12032004><FONT face=Arial color=#0000ff size=2>Since
the original release in '97 there has been only one submission of new data from
the AUC community (one compound by Geoff Howlett), and I believe that was in
'97. </FONT></SPAN></DIV>
<DIV><SPAN class=868041617-12032004><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=868041617-12032004><FONT face=Arial color=#0000ff size=2>If
anyone has any density or viscosity data for other compounds, even if they cover
only a limited range of concentrations, I'd be happy to calculate and circulate
the coefficients needed to add them to the database.</FONT></SPAN></DIV>
<DIV><SPAN class=868041617-12032004><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=868041617-12032004><FONT face=Arial color=#0000ff size=2>The
good news in your case is that it is probably reasonable to ignore the
contributions of 2 mM DTT. Any molecule of MW <= 200 at 2 mM is <= 0.4% by
weight in solution and thus probably won't change density or viscosity
significantly compared to the precision of your data. </FONT></SPAN></DIV>
<DIV><SPAN class=868041617-12032004><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=868041617-12032004><FONT face=Arial color=#0000ff size=2>John
Philo</FONT></SPAN></DIV>
<DIV><SPAN class=868041617-12032004><FONT face=Arial color=#0000ff
size=2>Alliance Protein Laboratories</FONT></SPAN></DIV>
<BLOCKQUOTE dir=ltr style="MARGIN-RIGHT: 0px">
<DIV></DIV>
<DIV class=OutlookMessageHeader lang=en-us dir=ltr align=left><FONT
face=Tahoma size=2>-----Original Message-----<BR><B>From:</B>
rasmb-admin@rasmb-email.bbri.org [mailto:rasmb-admin@rasmb-email.bbri.org]
<B>On Behalf Of </B>Samantha Jones (PRION)<BR><B>Sent:</B> Friday, March 12,
2004 5:44 AM<BR><B>To:</B> rasmb@rasmb-email.bbri.org<BR><B>Subject:</B>
[RASMB] DTT in SEDNTERP<BR><BR></FONT></DIV>
<DIV class=Section1><PRE><FONT face="Courier New" size=2><SPAN style="FONT-SIZE: 10pt">Hello<o:p></o:p></SPAN></FONT></PRE><PRE><FONT face="Courier New" size=2><SPAN style="FONT-SIZE: 10pt">I have the version of <SPAN class=SpellE>Sednterp</SPAN> from </SPAN></FONT><st1:date style="BACKGROUND-POSITION: left bottom; BACKGROUND-IMAGE: url(res://ietag.dll/#34/#1001); BACKGROUND-REPEAT: repeat-x" Year="2003" Day="2" Month="11">02/11/03</st1:date> and it does not have any<o:p></o:p></PRE><PRE><SPAN class=GramE><FONT face="Courier New" size=2><SPAN style="FONT-SIZE: 10pt">information</SPAN></FONT></SPAN> for DTT.<o:p></o:p></PRE><PRE><FONT face="Courier New" size=2><SPAN style="FONT-SIZE: 10pt">Can any help me get the parameters needed to include the 2mM DTT used in my buffer?<o:p></o:p></SPAN></FONT></PRE><PRE><FONT face="Courier New" size=2><SPAN style="FONT-SIZE: 10pt">Thanks<o:p></o:p></SPAN></FONT></PRE><PRE><FONT face="Courier New" size=2><SPAN style="FONT-SIZE: 10pt"><o:p> </o:p></SPAN></FONT></PRE><PRE><FONT face="Courier New" size=2><SPAN style="FONT-SIZE: 10pt"><o:p> </o:p></SPAN></FONT></PRE><PRE><FONT face="Courier New" size=2><SPAN style="FONT-SIZE: 10pt">Samantha Jones<o:p></o:p></SPAN></FONT></PRE><PRE><FONT face="Courier New" size=2><SPAN style="FONT-SIZE: 10pt"><o:p> </o:p></SPAN></FONT></PRE>
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