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<TITLE>Re: [RASMB] how large a species is practical?</TITLE>
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<BLOCKQUOTE>Hi Joel<BR>
<BR>
I endorse entirely what John Phil says. I would however have a certain preference for using the least squares g(s) from SEDFIT as a way of characterising the distribution, which from what you describe will be a continuous one. <BR>
<BR>
If all else fails, in respect of having enough time to acquire a decent data set, then adding (say) glycerol to increase the viscosity would work. But of course the guy with the sample might not want such an additive present! And if you are using interference optics then you should be able to grab plenty of data over a short time period in any case.<BR>
<BR>
Regards<BR>
<BR>
Arthur<BR>
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Arthur J Rowe<BR>
Professor of Biomolecular Technology<BR>
NCMH Business Centre<BR>
University of Nottingham<BR>
School of Biosciences<BR>
Sutton Bonington<BR>
Leicestershire LE12 5RD UK<BR>
<BR>
Tel: +44 (0)115 951 6156<BR>
+44 (0)116 271 4502<BR>
Fax: +44 (0)115 951 6157<BR>
email: arthur.rowe@nottingham.ac.uk<BR>
arthur.rowe@connectfree.co.uk (home)<BR>
Web: www.nottingham.ac.uk/ncmh/business<BR>
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hi all<BR>
I have had an industry chap come to me and want to explore the possibility <BR>
of using the AU as a means to check the quality and reproducibility of <BR>
preparations of an adjuvant (a mixture of stuff including saponins and <BR>
cholesterol that is added to antigens when injecting them - they apparently <BR>
help to promote an immune response to the antigen). The adjuvant forms <BR>
aggregates that appear to be of the order of 1 MDa - too big to be retained <BR>
on a sizing column.<BR>
My question is: am i likely to be able to record velocity data on such a <BR>
species. I realise that a quantitative analysis of the data may not be <BR>
feasible if these aggregates exhibit a wide range of sizes, but it may <BR>
simply be enough to discern differences between different preparations, for <BR>
his purposes.<BR>
<BR>
Have any of you worked on such large things before, and/or on heterogeneous <BR>
systems such as these? Any thoughts would be appreciated.<BR>
cheers<BR>
joel<BR>
<BR>
<BR>
<BR>
*****************************************************************<BR>
Dr Joel Mackay<BR>
NHMRC Senior Research Fellow<BR>
School of Molecular and Microbial Sciences<BR>
Building G08<BR>
University of Sydney,<BR>
Sydney, NSW 2006<BR>
Australia<BR>
ph +61-2-9351-3906<BR>
fax +61-2-9351-4726<BR>
WWW: http://www.mmb.usyd.edu.au/mackay/<BR>
Sydney Protein Group Website:<BR>
http://www.biochem.usyd.edu.au/spg/<BR>
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