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<DIV><SPAN class=459145716-23012004><FONT face=Arial size=2>Hello
all</FONT></SPAN></DIV>
<DIV><FONT face=Arial><FONT size=2><SPAN class=459145716-23012004>I have been
trying to analyse some interference data on Sedfit and have been repeatedly
seeing a</SPAN> <SPAN class=459145716-23012004>large 'half' peak at very
low s values on my s value distributions.</SPAN></FONT></FONT></DIV>
<DIV><FONT face=Arial><FONT size=2><SPAN class=459145716-23012004>On the
continuous c(s) distributions after fitting to range of 0.1s to 10s the
distribution line starts at c(s) values of 1.5 to 0.5 on 0 on the x axis,
dropping to 0 on the y axis by 0.1s value. This is still the case after
changing the parameters to 0.01s to 10s. </SPAN></FONT></FONT></DIV>
<DIV><FONT face=Arial><FONT size=2><SPAN class=459145716-23012004>The buffer
used at first was 10mM NaAcetate, 0.2% Azide and 2mM DTT pH 4. At first I
thought it could be the DTT but I saw the same thing using a sample in a buffer
10mM Tris 10mM Na Acetate, 0.2% Azide no DTT pH 8. </SPAN></FONT></FONT></DIV>
<DIV><FONT face=Arial><FONT size=2><SPAN class=459145716-23012004>I have seen
this spinning at 30K and 50K for about 16hours.</SPAN></FONT></FONT></DIV>
<DIV><FONT face=Arial><FONT size=2><SPAN
class=459145716-23012004></SPAN></FONT></FONT> </DIV>
<DIV><FONT face=Arial><FONT size=2><SPAN class=459145716-23012004>Could Azide be
the problem? I don't expect either sample to be contaminated, however anything
of this size would have been missed by the SDS-PAGE gel run on both
samples.</SPAN></FONT></FONT></DIV>
<DIV><FONT face=Arial><FONT size=2><SPAN
class=459145716-23012004></SPAN></FONT></FONT> </DIV>
<DIV><FONT face=Arial><FONT size=2><SPAN class=459145716-23012004>Thank
you</SPAN></FONT></FONT></DIV>
<DIV><FONT face=Arial><FONT size=2><SPAN
class=459145716-23012004></SPAN></FONT></FONT> </DIV>
<DIV><FONT face=Arial><FONT size=2><SPAN class=459145716-23012004>Samantha
Jones</SPAN></FONT></FONT></DIV>
<DIV><FONT face=Arial><FONT size=2><SPAN class=459145716-23012004><!-- Converted from text/rtf format -->
<P><SPAN lang=en-gb><I><FONT face="Palatino Linotype" size=2>Samantha
Jones</FONT></I></SPAN> <BR><SPAN lang=en-gb><I><FONT face="Palatino Linotype"
size=2>MRC Prion Unit</FONT></I></SPAN> <BR><SPAN lang=en-gb><I><FONT
face="Palatino Linotype" size=2>St Marys Hospital</FONT></I></SPAN> <BR><SPAN
lang=en-gb><I><FONT face="Palatino Linotype" size=2>Norfolk
Place</FONT></I></SPAN> <BR><SPAN lang=en-gb><I><FONT face="Palatino Linotype"
size=2>London</FONT></I></SPAN> <BR><SPAN lang=en-gb><I><FONT
face="Palatino Linotype" size=2>W2 1PG</FONT></I></SPAN>
</P></SPAN></FONT></FONT></DIV></BODY></HTML>