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<DIV><SPAN class=947024310-26112003><FONT face=Arial color=#0000ff size=2>Please
find a publication where we measured vbar of heparin: </FONT></SPAN></DIV><SPAN
class=947024310-26112003>
<P class=MsoNormal
style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 0cm; TEXT-ALIGN: left; mso-list: l118 level1 lfo198; tab-stops: list 21.3pt 53.25pt"
align=left><SPAN lang=EN-GB
style="FONT-SIZE: 10pt; mso-bidi-font-size: 12.0pt; mso-ansi-language: EN-GB"><FONT
face="Times New Roman">Pavlov G., Finet S., Tatarenko K., Korneeva E., Ebel
C.,<o:p></o:p></FONT></SPAN></P>
<P class=MsoNormal
style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; tab-stops: list 21.3pt"
align=left><SPAN lang=EN-GB
style="FONT-SIZE: 10pt; mso-bidi-font-size: 12.0pt; mso-ansi-language: EN-GB"><FONT
face="Times New Roman">Conformation of heparin studied with macromolecular
hydrodynamics methods and X-Rays scattering,<I
style="mso-bidi-font-style: normal"><o:p></o:p></I></FONT></SPAN></P>
<DIV><SPAN
style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-ansi-language: FR; mso-fareast-font-family: 'Times New Roman'; mso-fareast-language: FR; mso-bidi-language: AR-SA">(2003),<I
style="mso-bidi-font-style: normal"> Eur. Biophys. J</I>., <U>32</U>
437-449.</SPAN></DIV>
<DIV><SPAN
style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-ansi-language: FR; mso-fareast-font-family: 'Times New Roman'; mso-fareast-language: FR; mso-bidi-language: AR-SA"></SPAN> </DIV>
<DIV><SPAN
style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-ansi-language: FR; mso-fareast-font-family: 'Times New Roman'; mso-fareast-language: FR; mso-bidi-language: AR-SA"><SPAN
class=947024310-26112003>Extracted from the text:</SPAN></SPAN></DIV>
<DIV><SPAN
style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-ansi-language: FR; mso-fareast-font-family: 'Times New Roman'; mso-fareast-language: FR; mso-bidi-language: AR-SA"><SPAN
lang=EN-GB
style="FONT-SIZE: 12pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 10.0pt; mso-ansi-language: EN-GB; mso-fareast-font-family: 'Times New Roman'; mso-fareast-language: FR; mso-bidi-language: AR-SA">No
significant differences are found for heparin diluted in or dialysed against
0.2M NaCl and heparin diluted in H<SUB>2</SUB>O<SPAN class=947024310-26112003>
(20¢XC)</SPAN>. The derived buoyancy factor (</SPAN><SPAN lang=EN-GB
style="FONT-SIZE: 12pt; FONT-FAMILY: Symbol; mso-bidi-font-size: 10.0pt; mso-ansi-language: EN-GB; mso-fareast-font-family: 'Times New Roman'; mso-fareast-language: FR; mso-bidi-language: AR-SA; mso-bidi-font-family: 'Times New Roman'">Dr/D</SPAN><SPAN
lang=EN-GB
style="FONT-SIZE: 12pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 10.0pt; mso-ansi-language: EN-GB; mso-fareast-font-family: 'Times New Roman'; mso-fareast-language: FR; mso-bidi-language: AR-SA">c)
is 0.530</SPAN><SPAN lang=EN-GB
style="FONT-SIZE: 12pt; FONT-FAMILY: Symbol; mso-bidi-font-size: 10.0pt; mso-ansi-language: EN-GB; mso-fareast-font-family: 'Times New Roman'; mso-fareast-language: FR; mso-bidi-language: AR-SA; mso-bidi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-char-type: symbol; mso-symbol-font-family: Symbol"><SPAN
style="mso-char-type: symbol; mso-symbol-font-family: Symbol">¡Ó</SPAN></SPAN><SPAN
lang=EN-GB
style="FONT-SIZE: 12pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 10.0pt; mso-ansi-language: EN-GB; mso-fareast-font-family: 'Times New Roman'; mso-fareast-language: FR; mso-bidi-language: AR-SA">0.008,
corresponding to an apparent partial specific volume of 0.467 cm<SUP>3</SUP>/g,
which compares well with literature values: 0.50 in phosphate buffer, and 0.47
in NaCl buffer (Barlow et al. 1961; Lasker and Stivala 1966).
</SPAN></SPAN></SPAN></DIV>
<DIV><SPAN class=947024310-26112003><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=947024310-26112003>Hope
it can help</SPAN></FONT></DIV>
<P><FONT face=Arial size=2>Christine EBEL</FONT> <BR><FONT face=Arial
size=2>Institut de Biologie Structurale, UMR 5075 CEA-CNRS-UJF</FONT> <BR><FONT
face=Arial size=2>38 027 Grenoble Cedex 1, France.</FONT> <BR><FONT face=Arial
size=2>Tel: (33) 4 38 78 96 38 Fax: (33) 4 38 78 54
94</FONT> <BR><FONT face=Arial size=2>Email: Christine.Ebel@ibs.fr</FONT> </P>
<BLOCKQUOTE dir=ltr style="MARGIN-RIGHT: 0px">
<DIV class=OutlookMessageHeader dir=ltr align=left><FONT face=Tahoma
size=2>-----Message d'origine-----<BR><B>De :</B>
rasmb-admin@rasmb-email.bbri.org
[mailto:rasmb-admin@rasmb-email.bbri.org]<B>De la part de</B>
yandemin<BR><B>Envoyé :</B> mercredi 26 novembre 2003
10:51<BR><B>À :</B> rasmb@rasmb-email.bbri.org<BR><B>Objet :</B>
[RASMB] partial specific volume of a
glycosaminoglycan--"HEPARIN"<BR><BR></FONT></DIV>
<DIV><FONT size=2>
<DIV><FONT size=2>
<DIV><FONT size=5>Dear RASMBers,<BR><BR> Could anybody
kindly let me know the partial specific volume<BR>of a
glycosaminoglycan--"HEPARIN", or the place to look for? Protein-heparin
complex exp. use it. </FONT></DIV>
<DIV><FONT size=5>The 3D structure is too big as an appendix, and it is
on PDB, PDB number=1HPN, a fullysulfide-heparin. Can I estimate the V-bar
value?</FONT></DIV>
<DIV><FONT size=5></FONT> </DIV>
<DIV><FONT size=5> I would really appreciate any
help on this regard </FONT></DIV>
<DIV><FONT size=5> Thanks in
advance </FONT></DIV>
<DIV><FONT size=5>Best regards!</FONT></DIV>
<DIV><FONT
size=5> </FONT><FONT
size=5>Odysseus</FONT></DIV>
<DIV><FONT
size=5>
Department of Bioinformation</FONT></DIV>
<DIV><FONT
size=5> Graduate
School of Bioscience</FONT></DIV>
<DIV><FONT
size=5>
Tsing Hua University, Taiwan,
China</FONT></DIV></FONT></DIV></FONT></DIV></BLOCKQUOTE></BODY></HTML>