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Dear Christopher,
<p>thank you for testing our 6-ch. program. Unfortunately I had only 2
very old datasets of 6 ch. runs and was myself not quite sure because channal
ranges. I'll fix it in next time.
<p>About 'ctrl+prtscr' - it's commonly used of pc users. You have
to activate the SEGAL window with mouseclick and then push 'ctrl' and 'prtscr'
keys simultaneously. The picture is now copied on clipboard and you
can then simply paste it in some graphic program you have.
<p>I have one older version of SEGAL that can save fitted plots and resultes
in the spreadsheet file and I will put this option in the actual program.
<p>Also I'm thinking to make some add-on for SEGAL for Ka, etc. , and maybe
also the option for sedimentation velocity + diffusion...
<br>
<p>Best regards
<p>gia
<br>
<p>------------------------------------------------------------------------------
<p>Dr. Gia Machaidze
<br>Maurice E. Mueller Institute of Stuctural Biology
<br>Biozentrum/University of Basel
<br>Klingelbergstr. 70
<br>CH-4056 Basel/Switzerland
<p>Phone: +41 61 2672073
<br>Fax: +41 61 267 2109
<br>E. Mail: Gia.Machaidze@unibas.ch
<p>"Chin, Christopher" wrote:
<blockquote TYPE=CITE>
<p class="MsoNormal"><font face="Arial"><font size=-1>Dear Ariel, Gia and
colleagues,</font></font>
<p class="MsoNormal"><font face="Arial"><font size=-1> <?xml:namespace
prefix = o ns = "<A HREF="urn:schemas-microsoft-com:office:office">urn:schemas-microsoft-com:office:office</A>" /></font></font><o:p></o:p>
<p class="MsoNormal"><font face="Arial"><font size=-1>Thank you for the
update Segal program.<span
style="mso-spacerun: yes"></span>I was testing
your 6-channel update program and found that your radius region for each
channel is off calibration.<span style="mso-spacerun: yes"></span>I am
enclosed three raw data sets from Beckman 6 channel cells placed in an
An-60 Ti Rotor at three cell positions, ra1, ra2, ra3 for you to take a
look.<span
style="mso-spacerun: yes"></span>From my perspective this is
what I think is the correct channel window for all cells.</font></font>
<p class="MsoNormal"><o:p></o:p>
<br>
<table BORDER CELLSPACING=0 CELLPADDING=0 style="BORDER-BOTTOM: medium none; BORDER-COLLAPSE: collapse; BORDER-LEFT: medium none; BORDER-RIGHT: medium none; BORDER-TOP: medium none; mso-border-alt: solid windowtext .5pt; mso-padding-alt: 0in 5.4pt 0in 5.4pt" >
<caption><TBODY>
<br></TBODY></caption>
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<td VALIGN=TOP WIDTH="295" style="BORDER-BOTTOM: windowtext 0.5pt solid; BORDER-LEFT: windowtext 0.5pt solid; BORDER-RIGHT: windowtext 0.5pt solid; BORDER-TOP: windowtext 0.5pt solid; PADDING-BOTTOM: 0in; PADDING-LEFT: 5.4pt; PADDING-RIGHT: 5.4pt; PADDING-TOP: 0in; WIDTH: 221.4pt">
<center><span
style="FONT-SIZE: 14pt; mso-bidi-font-size: 12.0pt"><b><i>Cell </i></b><o:p></o:p></span></center>
</td>
<td VALIGN=TOP WIDTH="295" style="BORDER-BOTTOM: windowtext 0.5pt solid; BORDER-LEFT: medium none; BORDER-RIGHT: windowtext 0.5pt solid; BORDER-TOP: windowtext 0.5pt solid; PADDING-BOTTOM: 0in; PADDING-LEFT: 5.4pt; PADDING-RIGHT: 5.4pt; PADDING-TOP: 0in; WIDTH: 221.4pt; mso-border-left-alt: solid windowtext .5pt">
<h1>
Radius window<span
style="FONT-STYLE: normal; FONT-WEIGHT: normal"><o:p></o:p></span></h1>
</td>
</tr>
<tr>
<td VALIGN=TOP WIDTH="295" style="BORDER-BOTTOM: windowtext 0.5pt solid; BORDER-LEFT: windowtext 0.5pt solid; BORDER-RIGHT: windowtext 0.5pt solid; BORDER-TOP: medium none; PADDING-BOTTOM: 0in; PADDING-LEFT: 5.4pt; PADDING-RIGHT: 5.4pt; PADDING-TOP: 0in; WIDTH: 221.4pt; mso-border-top-alt: solid windowtext .5pt">
<center><span
style="FONT-SIZE: 14pt; mso-bidi-font-size: 12.0pt"><b><i>Channel
1</i></b><o:p></o:p></span></center>
</td>
<td VALIGN=TOP WIDTH="295" style="BORDER-BOTTOM: windowtext 0.5pt solid; BORDER-LEFT: medium none; BORDER-RIGHT: windowtext 0.5pt solid; BORDER-TOP: medium none; PADDING-BOTTOM: 0in; PADDING-LEFT: 5.4pt; PADDING-RIGHT: 5.4pt; PADDING-TOP: 0in; WIDTH: 221.4pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt">
<center><span
lang=FR
style="FONT-SIZE: 14pt; mso-bidi-font-size: 12.0pt; mso-ansi-language: FR"><b><i>5.8-6.2</i></b><o:p></o:p></span>
<p><span lang=FR
style="FONT-SIZE: 14pt; mso-bidi-font-size: 12.0pt; mso-ansi-language: FR"><o:p></o:p></span></center>
</td>
</tr>
<tr>
<td VALIGN=TOP WIDTH="295" style="BORDER-BOTTOM: windowtext 0.5pt solid; BORDER-LEFT: windowtext 0.5pt solid; BORDER-RIGHT: windowtext 0.5pt solid; BORDER-TOP: medium none; PADDING-BOTTOM: 0in; PADDING-LEFT: 5.4pt; PADDING-RIGHT: 5.4pt; PADDING-TOP: 0in; WIDTH: 221.4pt; mso-border-top-alt: solid windowtext .5pt">
<center><span
lang=FR
style="FONT-SIZE: 14pt; mso-bidi-font-size: 12.0pt; mso-ansi-language: FR"><b><i>Channel
2</i></b><o:p></o:p></span></center>
</td>
<td VALIGN=TOP WIDTH="295" style="BORDER-BOTTOM: windowtext 0.5pt solid; BORDER-LEFT: medium none; BORDER-RIGHT: windowtext 0.5pt solid; BORDER-TOP: medium none; PADDING-BOTTOM: 0in; PADDING-LEFT: 5.4pt; PADDING-RIGHT: 5.4pt; PADDING-TOP: 0in; WIDTH: 221.4pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt">
<center><span
lang=FR
style="FONT-SIZE: 14pt; mso-bidi-font-size: 12.0pt; mso-ansi-language: FR"><b><i>6.2-6.7</i></b><o:p></o:p></span>
<p><span lang=FR
style="FONT-SIZE: 14pt; mso-bidi-font-size: 12.0pt; mso-ansi-language: FR"><o:p></o:p></span></center>
</td>
</tr>
<tr>
<td VALIGN=TOP WIDTH="295" style="BORDER-BOTTOM: windowtext 0.5pt solid; BORDER-LEFT: windowtext 0.5pt solid; BORDER-RIGHT: windowtext 0.5pt solid; BORDER-TOP: medium none; PADDING-BOTTOM: 0in; PADDING-LEFT: 5.4pt; PADDING-RIGHT: 5.4pt; PADDING-TOP: 0in; WIDTH: 221.4pt; mso-border-top-alt: solid windowtext .5pt">
<center><span
lang=FR
style="FONT-SIZE: 14pt; mso-bidi-font-size: 12.0pt; mso-ansi-language: FR"><b><i>Channel
3</i></b><o:p></o:p></span></center>
</td>
<td VALIGN=TOP WIDTH="295" style="BORDER-BOTTOM: windowtext 0.5pt solid; BORDER-LEFT: medium none; BORDER-RIGHT: windowtext 0.5pt solid; BORDER-TOP: medium none; PADDING-BOTTOM: 0in; PADDING-LEFT: 5.4pt; PADDING-RIGHT: 5.4pt; PADDING-TOP: 0in; WIDTH: 221.4pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt">
<center><span
lang=FR
style="FONT-SIZE: 14pt; mso-bidi-font-size: 12.0pt; mso-ansi-language: FR"><b><i>6.7-7.15</i></b><o:p></o:p></span>
<p><span lang=FR
style="FONT-SIZE: 14pt; mso-bidi-font-size: 12.0pt; mso-ansi-language: FR"><o:p></o:p></span></center>
</td>
</tr>
</table>
<p class="MsoNormal"><span lang=FR style="mso-ansi-language: FR"><span
style="mso-spacerun: yes"></span><o:p></o:p></span>
<p class="MsoNormal"><span lang=FR
style="mso-ansi-language: FR"><o:p></o:p></span>
<p class="MsoNormal"><font face="Arial"><font size=-1>I also test your
page-copy function using control, print screen. I could not get it to work.<span style="mso-spacerun: yes"></span>However
I remember John Philo DCDT+ program, he told me to use <b>control insert
to copy</b> and then use <b>control V</b> <b>to paste </b>the data into
Microsoft word for record keeping (I like to use Microsoft word to organized
my experimental data).</font></font>
<p class="MsoNormal"><o:p></o:p>
<p class="MsoNormal"><font face="Arial"><font size=-1>I am looking forward
for your next update so we can use Segal program to get Ka value.</font></font>
<p class="MsoNormal"><font face="Arial"><font size=-1>I like to thank you
and your partner Gia Machaidze in advance for your dedication and determination
to make AUC data analysis more enjoyable and also easy to use.</font></font>
<p class="MsoNormal"><o:p></o:p>
<p class="MsoNormal"><font face="Arial"><font size=-1>With regards,</font></font>
<p class="MsoNormal"><o:p></o:p>
<p class="MsoNormal"><font face="Arial"><font size=-1>Chris</font></font> <font face="Arial"><font size=-1>--------------------------------------------------------------------------</font></font>
<br><font face="Arial"><font size=-1>Christopher Chin</font></font>
<br><font face="Arial"><font size=-1>Manager, XLA-Analytical Ultracentrifugation
facility</font></font>
<br><font face="Arial"><font size=-1>Sealy Center for Structural Biology</font></font>
<br><font face="Arial"><font size=-1>HBC&G, 5.134 MRB.UTMB, Galveston,Tx
77555-1055</font></font>
<br><font face="Arial"><font size=-1>cchin@utmb.edu, 409-772-1693, efax
708-585-1920</font></font>
<br><font face="Arial"><font size=-1>---------------------------------------------------------------------------</font></font> </blockquote>
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