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<P class=MsoNormal>Dear Ariel, Gia and colleagues,</P>
<P class=MsoNormal> <?xml:namespace prefix = o ns =
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<P class=MsoNormal>Thank you for the update Segal program.<SPAN
style="mso-spacerun: yes"> </SPAN>I was testing your 6-channel update
program and found that your radius region for each channel is off
calibration.<SPAN style="mso-spacerun: yes"> </SPAN>I am enclosed three
raw data sets from Beckman 6 channel cells placed in an An-60 Ti Rotor at three
cell positions, ra1, ra2, ra3 for you to take a look.<SPAN
style="mso-spacerun: yes"> </SPAN>From my perspective this is what I think
is the correct channel window for all cells.</P>
<P class=MsoNormal> <o:p></o:p></P>
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<P align=center class=MsoNormal style="TEXT-ALIGN: center"><B><I><SPAN
style="FONT-SIZE: 14pt; mso-bidi-font-size: 12.0pt">Cell
<o:p></o:p></SPAN></I></B></P></TD>
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<H1>Radius window<SPAN
style="FONT-STYLE: normal; FONT-WEIGHT: normal"><o:p></o:p></SPAN></H1></TD></TR>
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<P align=center class=MsoNormal style="TEXT-ALIGN: center"><B><I><SPAN
style="FONT-SIZE: 14pt; mso-bidi-font-size: 12.0pt">Channel
1<o:p></o:p></SPAN></I></B></P></TD>
<TD
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<P align=center class=MsoNormal style="TEXT-ALIGN: center"><B><I><SPAN
lang=FR
style="FONT-SIZE: 14pt; mso-bidi-font-size: 12.0pt; mso-ansi-language: FR">5.8-6.2<o:p></o:p></SPAN></I></B></P>
<P align=center class=MsoNormal
style="TEXT-ALIGN: center"> <B><I><SPAN lang=FR
style="FONT-SIZE: 14pt; mso-bidi-font-size: 12.0pt; mso-ansi-language: FR"><o:p></o:p></SPAN></I></B></P></TD></TR>
<TR>
<TD
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vAlign=top width=295>
<P align=center class=MsoNormal style="TEXT-ALIGN: center"><B><I><SPAN
lang=FR
style="FONT-SIZE: 14pt; mso-bidi-font-size: 12.0pt; mso-ansi-language: FR">Channel
2<o:p></o:p></SPAN></I></B></P></TD>
<TD
style="BORDER-BOTTOM: windowtext 0.5pt solid; BORDER-LEFT: medium none; BORDER-RIGHT: windowtext 0.5pt solid; BORDER-TOP: medium none; PADDING-BOTTOM: 0in; PADDING-LEFT: 5.4pt; PADDING-RIGHT: 5.4pt; PADDING-TOP: 0in; WIDTH: 221.4pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt"
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<P align=center class=MsoNormal style="TEXT-ALIGN: center"><B><I><SPAN
lang=FR
style="FONT-SIZE: 14pt; mso-bidi-font-size: 12.0pt; mso-ansi-language: FR">6.2-6.7<o:p></o:p></SPAN></I></B></P>
<P align=center class=MsoNormal
style="TEXT-ALIGN: center"> <B><I><SPAN lang=FR
style="FONT-SIZE: 14pt; mso-bidi-font-size: 12.0pt; mso-ansi-language: FR"><o:p></o:p></SPAN></I></B></P></TD></TR>
<TR>
<TD
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vAlign=top width=295>
<P align=center class=MsoNormal style="TEXT-ALIGN: center"><B><I><SPAN
lang=FR
style="FONT-SIZE: 14pt; mso-bidi-font-size: 12.0pt; mso-ansi-language: FR">Channel
3<o:p></o:p></SPAN></I></B></P></TD>
<TD
style="BORDER-BOTTOM: windowtext 0.5pt solid; BORDER-LEFT: medium none; BORDER-RIGHT: windowtext 0.5pt solid; BORDER-TOP: medium none; PADDING-BOTTOM: 0in; PADDING-LEFT: 5.4pt; PADDING-RIGHT: 5.4pt; PADDING-TOP: 0in; WIDTH: 221.4pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt"
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<P align=center class=MsoNormal style="TEXT-ALIGN: center"><B><I><SPAN
lang=FR
style="FONT-SIZE: 14pt; mso-bidi-font-size: 12.0pt; mso-ansi-language: FR">6.7-7.15<o:p></o:p></SPAN></I></B></P>
<P align=center class=MsoNormal
style="TEXT-ALIGN: center"> <B><I><SPAN lang=FR
style="FONT-SIZE: 14pt; mso-bidi-font-size: 12.0pt; mso-ansi-language: FR"><o:p></o:p></SPAN></I></B></P></TD></TR></TBODY></TABLE>
<P class=MsoNormal><SPAN lang=FR style="mso-ansi-language: FR"><SPAN
style="mso-spacerun: yes"> </SPAN><o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN lang=FR
style="mso-ansi-language: FR"> <o:p></o:p></SPAN></P>
<P class=MsoNormal>I also test your page-copy function using control, print
screen. I could not get it to work.<SPAN style="mso-spacerun: yes">
</SPAN>However I remember John Philo DCDT+ program, he told me to use <B>control
insert to copy</B> and then use <B>control V</B> <B>to paste </B>the data into
Microsoft word for record keeping (I like to use Microsoft word to organized my
experimental data). </P>
<P class=MsoNormal> <o:p></o:p></P>
<P class=MsoNormal>I am looking forward for your next update so we can use Segal
program to get Ka value.</P>
<P class=MsoNormal>I like to thank you and your partner Gia Machaidze in advance
for your dedication and determination to make AUC data analysis more enjoyable
and also easy to use.</P>
<P class=MsoNormal> <o:p></o:p></P>
<P class=MsoNormal>With regards,</P>
<P class=MsoNormal> <o:p></o:p></P>
<P class=MsoNormal>Chris</P></FONT></FONT></DIV>
<DIV> </DIV>
<DIV> </DIV>
<DIV> </DIV>
<DIV> </DIV>
<DIV> <FONT face=Arial
size=2>--------------------------------------------------------------------------</FONT>
<BR><FONT face=Arial size=2>Christopher Chin</FONT> <BR><FONT face=Arial
size=2>Manager, XLA-Analytical Ultracentrifugation facility</FONT> <BR><FONT
face=Arial size=2>Sealy Center for Structural Biology</FONT> <BR><FONT
face=Arial size=2>HBC&G, 5.134 MRB.UTMB, Galveston,Tx 77555-1055</FONT>
<BR><FONT face=Arial size=2>cchin@utmb.edu, 409-772-1693, efax
708-585-1920</FONT> <BR><FONT face=Arial
size=2>---------------------------------------------------------------------------</FONT>
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