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<TITLE>Re: [RASMB] 2 ligands and no equation</TITLE>
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Holger -<BR>
<BR>
Happy New Year to you and all RASMBers !<BR>
<BR>
As regards your problem, it seems to me that doing maths on dynamic processes is boring - and hardly necessary when there is plenty of software which "is designed to facilitate the mapping, modeling and simulating of dynamic processes". To quote from the web site of those who make and market my own favoured product (STELLA, from High Performance Systems, Inc: http://www.hps-inc.com/index.htm, click on STELLA in the LH menu). <BR>
<BR>
Of course there are other programs out there, but STELLA is long established, is both Mac and PC compatible (and models can be transferred across the platforms), and does not take lots of learning to get output. Although a lot of its applications are social science based, a rate is a rate, and it's easy to set a model* up for a multi-component interacting system.<BR>
<BR>
Just a thought<BR>
<BR>
Arthur<BR>
<BR>
e.g. Alistair McGregor, Andrew D Blanchard, Arthur J Rowe and David R Critchley (1994) Identification of the vinculin binding site in the cytoskeletal protein a-actinin Biochem J <B>301</B> 225-233<BR>
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Arthur J Rowe<BR>
Professor of Biomolecular Technology<BR>
NCMH Business Centre<BR>
University of Nottingham<BR>
School of Biosciences<BR>
Sutton Bonington<BR>
Leicestershire LE12 5RD UK<BR>
<BR>
Tel: +44 (0)115 951 6156<BR>
+44 (0)116 271 4502<BR>
Fax: +44 (0)115 951 6157<BR>
email: arthur.rowe@nottingham.ac.uk<BR>
arthur.rowe@connectfree.co.uk (home)<BR>
Web: www.nottingham.ac.uk/ncmh/business<BR>
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<BLOCKQUOTE><B>From: </B>Holger Strauss <strauss@fmp-berlin.de><BR>
<B>Date: </B>Mon, 13 Jan 2003 11:47:43 +0100 (MET)<BR>
<B>To: </B>mail-to-all at rasmb <rasmb@rasmb-email.bbri.org><BR>
<B>Subject: </B>[RASMB] 2 ligands and no equation<BR>
<BR>
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Hello everbody,<BR>
<BR>
the new year has hardly begun, the institute slowly gets back to live, and<BR>
we are stuck with the following problem: we have a protein and two<BR>
ligands, both binding to the same binding site on the protein. We know<BR>
both the individual Kds (mikromolar range) of the ligands binding to the<BR>
protein. Now, the question is, how can<BR>
one describe the concentrations of all species (bound/free ligand A,<BR>
bound/free ligand B, free protein), when both ligands are<BR>
present at about equal concentrations (+- one order of magnitude) together<BR>
with the protein? Our efforts so far landed us with some horrifying<BR>
equations with an equally horrifying number of solutions... but there must<BR>
be a solution to the problem, must there not? All comments are greatly<BR>
appreciated!<BR>
<BR>
Greeting and happy New Year to all, Holger<BR>
<BR>
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - <BR>
<BR>
Holger Strauss <BR>
<BR>
Forschungsinstitut fuer Molekulare Pharmakologie (FMP)<BR>
Robert-Roessle Strasse 10<BR>
<BR>
13125 Berlin/Germany<BR>
<BR>
Tel: +49 (0)30 94793 - 223 (office)<BR>
- 316 (lab)<BR>
<BR>
Fax: +49 (0)30 94793 - 169 <BR>
<BR>
<BR>
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - <BR>
<BR>
Science is spectrum analysis; art is photosynthesis.<BR>
<BR>
Karl Kraus<BR>
<BR>
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