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<DIV><FONT face=Arial size=2>I am presently studying the binding of a 58900
dalton coagulation protein to a 2676 dalton peptide. I have performed
velocity runs to determine the sedimentation coefficients of each protein.
My next goal is to perform an equilibrium run but I am not sure how many
diffrent concentrations I must prepare as well as what diffrent speeds to carry
out the run. I also would like to know if AUC is a good method to
assess binding between two proteins with a large gap in molecular weights?
Can the results be analyzed using the NONLIN program and will I be able to
determine the binding constant Kd?</FONT></DIV>
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<DIV><FONT face=Arial size=2>Thank you</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>Caterina Cammalleri</FONT></DIV></BODY></HTML>