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<DIV><SPAN class=750012000-14122001><FONT face=Arial color=#0000ff
size=2>Kate,</FONT></SPAN></DIV>
<DIV><SPAN class=750012000-14122001><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=750012000-14122001><FONT face=Arial color=#0000ff size=2>There
are two potential causes for this problem: software and
hardware.</FONT></SPAN></DIV>
<DIV><SPAN class=750012000-14122001><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=750012000-14122001><FONT face=Arial color=#0000ff size=2>In
velocity runs, it is true that normally the delay calibration causes the
monochromator to move prior to the first scan, but the software is not
supposed to instruct the monochromator to move during subsequent scans at the
same wavelength. However, at least in some versions of the firmware in the
instrument that hasn't been true, and it keeps telling the instrument to 'seek'
the desired wavelength prior to each scan, causing the wavelength to fluctuate
as a consequence of this hunting. I haven't heard of such problems with recent
firmware, though. FYI I was told earlier this year that Beckman was going to
change the firmware and have it use the measurement wavelength for the delay
setting, but I don't know whether this has been implemented
yet.</FONT></SPAN></DIV>
<DIV><SPAN class=750012000-14122001><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=750012000-14122001><FONT face=Arial color=#0000ff size=2>My
instrument sometimes exhibits a phenomenon similar to yours, but it appears to
be a hardware issue. In my case, usually there is a single shift in wavelength
at some point during the run.</FONT></SPAN></DIV>
<DIV><SPAN class=750012000-14122001><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=750012000-14122001><FONT face=Arial color=#0000ff size=2>What I
believe happens is that there is slow 'creep' of the position of
the post that moves the monochromator. On my instrument (a very old one)
the theory was that the o-ring that forms the vacuum seal on that post had
gotten sticky. We did replace that and that may have reduced the rate of
incidence of this problem, but it still recurs occasionally. Possibly
temperature fluctuations could cause some motion too? Anyway, I do see this
sometimes (not always showing as fluctuations in the wavelength recorded in the
header, but sometimes). </FONT></SPAN></DIV><SPAN
class=750012000-14122001></SPAN><FONT face=Tahoma><FONT face=Arial color=#0000ff
size=2></FONT><FONT face=Arial color=#0000ff size=2></FONT><FONT face=Arial
color=#0000ff size=2></FONT><FONT face=Arial color=#0000ff size=2></FONT><FONT
face=Arial color=#0000ff size=2></FONT><FONT face=Arial color=#0000ff
size=2></FONT><FONT face=Arial color=#0000ff size=2></FONT><FONT face=Arial
color=#0000ff size=2></FONT><FONT face=Arial color=#0000ff size=2></FONT><FONT
face=Arial color=#0000ff size=2></FONT>
<DIV><BR></DIV>
<DIV><FONT size=2><SPAN class=750012000-14122001><FONT face=Arial
color=#0000ff>So you might have your service engineer at least change and
lubricate that o-ring, and perhaps check out the whole motor-pot
assembly.</FONT></SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=750012000-14122001></SPAN></FONT> </DIV>
<DIV><FONT size=2><SPAN class=750012000-14122001><FONT face=Arial
color=#0000ff>John Philo </FONT></SPAN></FONT></DIV>
<DIV><FONT size=2><SPAN class=750012000-14122001></SPAN></FONT> </DIV>
<DIV><FONT size=2><SPAN class=750012000-14122001> </SPAN>-----Original
Message-----<BR><B>From:</B> rasmb-admin@rasmb-email.bbri.org
[mailto:rasmb-admin@rasmb-email.bbri.org]<B>On Behalf Of </B>Katharina
Spiegel<BR><B>Sent:</B> Thursday, December 13, 2001 11:27 AM<BR><B>To:</B>
rasmb@server1.bbri.org<BR><B>Subject:</B> [RASMB] XLA NEW Jumping wavelength
problem<BR><BR></DIV></FONT></FONT>
<BLOCKQUOTE dir=ltr style="MARGIN-RIGHT: 0px">
<DIV>Dear RASMB Colleagues,</DIV>
<DIV><FONT face=Arial color=#0000ff size=2></FONT><FONT face=Arial
color=#0000ff size=2></FONT><BR></DIV>
<DIV>I am encountering a phenomenon with our XLA which hopefully one of you
has seen already and can help me out with practical advice.</DIV>
<DIV><FONT face=Arial color=#0000ff size=2></FONT><FONT face=Arial
color=#0000ff size=2></FONT><BR></DIV>
<DIV>We started to see a change in wavelength during velocity scanning despite
setting the instrument to continuous scanning of one single wavelength only.
Admittedly, "starting to see" means actually that we "started to look", so I
don't know exactly for how long we have had this problem. Please view the
attached scan records. Our service engineer remains undisturbed, since he
considers these changes "within specs", but pressed harder for an
explanation/solution he inquired internally and came back with the following
explanation:</DIV>
<DIV><FONT face=Arial color=#0000ff size=2></FONT><FONT face=Arial
color=#0000ff size=2></FONT><BR></DIV>
<DIV>Since the delay calibration was not switched off the instrument performs
it prior to each scan thereby routinely moving the monochrometer to
500nm. Switching back and forth between 500nm and our scan wavelength of 220nm
is then causing the variation. He suggested to speed up the rotor to the
desired speed and run the delay calibration once, switch it then off (check NO
DELAY CALIBRATION under options) and then "start the run" Obviously, this
approach needed to be altered to 1) fill rotor with mock filled cell and
counterbalance 2) spin it up to run speed and acquire one scan with delay
calibration 3) stop XLA and reload rotor with sample cells 4) program run
conditions with delay calibration switched off 5) start velocity run.</DIV>
<DIV><FONT face=Arial color=#0000ff size=2></FONT><FONT face=Arial
color=#0000ff size=2></FONT><FONT face=Arial color=#0000ff
size=2></FONT><BR></DIV>
<DIV>Short of time I did it this way for my most recent velocity run, and the
problem nevertheless remained. I take this as further evidence that there is a
instrument problem -one which my service engineer apparently has not yet seen
or at least is reluctant to acknowledge as a problem. Therefore, I am looking
for something to tip him off as to what he should be looking for.</DIV>
<DIV><FONT face=Arial color=#0000ff size=2></FONT><FONT face=Arial
color=#0000ff size=2></FONT><BR></DIV>
<DIV>I truly hope some of you have some ideas/suggestions what to do about
this.</DIV>
<DIV><FONT face=Arial color=#0000ff size=2></FONT><FONT face=Arial
color=#0000ff size=2></FONT><BR></DIV>
<DIV>Kind regards,</DIV>
<DIV><FONT face=Arial color=#0000ff size=2></FONT><BR></DIV>
<DIV>Kate</DIV>
<DIV><BR></DIV>
<DIV><BR></DIV>
<DIV><BR></DIV>
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220 1</BLOCKQUOTE>
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<DIV><BR></DIV>
<DIV>--
<BR>*****************************************<SPAN></SPAN>************************<BR>Katharina
Spiegel, Ph.D. <X-TAB>
</X-TAB><X-TAB> </X-TAB>Research
Assistant Professor<X-TAB>
</X-TAB><X-TAB>
</X-TAB><BR>Off:<X-TAB>
</X-TAB>847-467-6533<X-TAB>
</X-TAB><X-TAB> </X-TAB>Manager,
Keck Biophysics Facility<BR>Lab:<X-TAB>
</X-TAB>847-467-6534<X-TAB>
</X-TAB><X-TAB> </X-TAB>Northwestern
University, Dept.
BMBCB<BR>Fax:<X-TAB>
</X-TAB>847-467-6489<X-TAB>
</X-TAB><X-TAB> </X-TAB>2153 N.
Sheridan Road<BR>Email: k-spiegel@northwestern.edu<X-TAB>
</X-TAB>Evanston, IL 60208
USA<BR><BR>http://www.biochem.northwestern.edu/Keck/<SPAN></SPAN>keckmain.html<BR><BR>*****************************************<SPAN></SPAN>************************<BR></DIV></BLOCKQUOTE></BODY></HTML>