[RASMB] 答复: RASMB Digest, Vol 92, Issue 1

[Chen Mengdi]

Dear all, 

I double checked my lab journal and found out the problem rose up after a run with a 70% sucrose sample. I would guess the Epon cell was swelling after exposing the such high concentration of sucrose. I have tried to soak the Epon centerpiece in water overnight. It seemed to help a bit. I plan to extend the soaking time to several days to see if the swelling can be recovered. Many thanks for your kind replys :)

Bests,
Mengdi

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发送时间: 2022年10月28日 4:05
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主题: RASMB Digest, Vol 92, Issue 1

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Today's Topics:

   1. Epon centerpiece (Chen Mengdi)


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Message: 1
Date: Thu, 27 Oct 2022 08:59:58 +0000
From: Chen Mengdi <chenmengdidi at outlook.com>
To: "rasmb at list.rasmb.org" <rasmb at list.rasmb.org>
Subject: [RASMB] Epon centerpiece
Message-ID:
	<DB6PR0202MB26936EABB84C85BD04261BD3DD339 at DB6PR0202MB2693.eurprd02.prod.outlook.com>
	
Content-Type: text/plain; charset="us-ascii"

Dear all,

I ordered an AUC cell assembly from Beckman which contained an Epon centerpiece. But only after a few runs, the cell was leaking even without vacuum. I found that the centerpiece cannot be pushed smoothly to the bottom of the cell when I assembled the cell.

Has anyone of you met this similar problem? Any there any suggestions for solving this problem? Shall I use an O ring on top of the centerpiece to seal the cell, like how we assemble the Al centerpiece?

Many thanks.

Best wishes,
Mengdi Chen
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