[RASMB] Optima AUC Interference optics question

[Borries Demeler]

Take a look at the original images, there is probably a black spot at this
position somewhere in the image. My guess is you have dirt in the optics or
dead pixels in the camera. This noise  offsets the Fourier transform and
you get random fringe shifts. If the noise streaks through the entire image
at the same radial position it is likely just dirt. Carefully inspect the
top of the camera and see if you can see anything stuck on the top and
remove it carefully. Anything below that should be addressed by Beckman
service.
-Borries




On Fri, Apr 30, 2021 at 9:46 PM Jeff Arthur <jarthur at kbibiopharma.com>
wrote:

> Hi Everyone,
>
>
>
> I am having an issue with the interference optics on my Optima and I am
> hoping to get any advice on how to troubleshoot.  The issue is screenshot
> below, which shows two successive scans on each of two cells. My last
> couple runs have a blip at ~6.5 cm.  It is present in every cell, so I
> assume that it is instrument related.  At radial positions lower than the
> blip, the fringe shift is inconsistent between successive scans, which is
> creating difficulties in analyzing the results, even if the region
> immediately surrounding 6.5 cm is excluded from the raw data.  It’s
> probably coincidental,  but I also noticed that the revolution counter
> decreased significantly immediately before this issue cropped up.  Before
> the issue it was ~550,000, and not it is ~450,000.
>
>
>
> Thanks for the help!
>
> Jeff
>
>
>
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