[RASMB] s value and classical sucrose gradient separation

[John Sumida]

Dear RASMB,

 

How would one use the sedimentation value to design a classical sucrose
gradient experiment to extract and then purify a species of interest?

 

This question is in relation to the characterization and isolation of
nanoparticles.

 

I imagine it would be using the observed sedimentation value obtained
experimentally and calculating r to determine the radius at a given buoyance
ratio as per

 



Hirst_Cox_Biochem_J_1976_159 (see attached).

 

However, if there is a standard protocol in current use, I would be
interested in a reference if appropriate.  

 

Thank you for considering my question.

 

Best regards,

John Sumida

Molecular Analysis Facility

University of Washington

 

 

 

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