[RASMB] [EXTERNAL] Serum and EH&S

[John Sumida]

Dear Susumu,

 

Thank you for your response.  Interesting that your facility does accept
third party testing of human serum samples.  I will be meeting with our EH&S
group next week and this information together with John's will be helpful.
I assume that you are running your AUC under standard BS-L1 conditions, and
that it is sufficient for you to show that your samples have been tested for
blood borne pathogens?

 

Once again, thank you for taking the time to respond to my question.

 

Best regards,

John Sumida

University of Washington.

 

From: RASMB [mailto:rasmb-bounces at list.rasmb.org] On Behalf Of Susumu
UCHIYAMA
Sent: Thursday, September 27, 2018 12:34 PM
To: rasmb at list.rasmb.org
Subject: Re: [RASMB] [EXTERNAL] Serum and EH&S

 

We also run AUC of proteins in serum using commercial human and murine serum
(ref attached). Commercial human serum is usually tested for infectious
material before selling but it might need higher level of bio-safety if you
use serum from patients. 
As for serum from health volunteer, it depends on the guideline of the
organization.

I guess AUC has filter not HEPA but "BioSafe optional dual PALL
Pharmaceutical-Grade Sterilizing Filters" which is installed for preparative
UC at the vacuum exhaust. 

Best,
Susumu




From: RASMB [mailto:rasmb-bounces at list.rasmb.org] On Behalf Of John Sumida
Sent: Wednesday, September 26, 2018 5:00 PM
To: RASMB  <mailto:rasmb at list.rasmb.org> <rasmb at list.rasmb.org>
Subject: [EXTERNAL] [RASMB] Serum and EH&S

 

Dear RASMB,

 

I apologize that this is not a scientific question, but this is, perhaps, a
question that has come up for some on the list and I would be curious to
hear how it was handled. 

 

We periodically field requests to spin human serum solutions, which
invariably is a health & safety problem due to the potential for a cell leak
and the subsequent aerosolization of a potentially hazardous material into
the lab environment.  

 

As an academic lab, our resources and institutional motivation in
engineering a space specific for this application are limited.  Thus, one
suggestion was to use third party testing of samples being submitted for
analysis.  However this is costly and most users have abandoned their
efforts to perform these measurements as a result.

 

I would be curious to know if third party testing of potentially infectious
materials such as human serum is commonly used within an EH&S setting, or if
it is possible to place a HEPA filter in the vacuum exhaust if the
instrument uses a TMP?

 

Thank you in advance for any information you may be able to offer.

 

Best regards

John Sumida

University of Washington.

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