[RASMB] [EXTERNAL] Serum and EH&S

[John Sumida]

Dear John,

 

Thank you for your response to my question.  Interesting that your
institution accepts third party testing of human serum samples.  Ours does
not currently have any formal policy in place but has taken the position
that it is not possible to test for all potential blood borne pathogens.

 

With the older diffusion pump based instrument, I think doing these
measurements was more feasible since one did not have to worry about
damaging the TMP?

 

Do you have any recommended literature to point me at with respect to
measuring the hydrodynamic scaling constant (ks) or the second viral
coefficient (BMI)?  Do you do the latter by DLS or Sed Eq?

 

Thanks again for your response, and I hope that next time I post on this
topic I will have more interesting questions and some actual data to query
the group about.

 

Best regards,

John Sumida

University of Washington

From: RASMB [mailto:rasmb-bounces at list.rasmb.org] On Behalf Of John J.
Correia
Sent: Thursday, September 27, 2018 11:58 AM
To: John Sumida <jpsumida at uw.edu>; RASMB <rasmb at list.rasmb.org>
Subject: Re: [RASMB] [EXTERNAL] Serum and EH&S

 

John, my lab has been doing human serum proteins and human serum runs
without concerns (see attached for relevant studies). When you buy human
serum it's been tested for infectious material. The normal precautions of
wearing gloves, a face mask, and then cleaning cell components are
encouraged. Interpretation of the data is a bit harder since you must
include ks and BM1 cross term values and thus know something about the serum
composition and its interaction with your system of interest. This is part
of the ongoing part of our work in this area.

 

From: RASMB [mailto:rasmb-bounces at list.rasmb.org] On Behalf Of John Sumida
Sent: Wednesday, September 26, 2018 5:00 PM
To: RASMB <rasmb at list.rasmb.org <mailto:rasmb at list.rasmb.org> >
Subject: [EXTERNAL] [RASMB] Serum and EH&S

 

Dear RASMB,

 

I apologize that this is not a scientific question, but this is, perhaps, a
question that has come up for some on the list and I would be curious to
hear how it was handled. 

 

We periodically field requests to spin human serum solutions, which
invariably is a health & safety problem due to the potential for a cell leak
and the subsequent aerosolization of a potentially hazardous material into
the lab environment.  

 

As an academic lab, our resources and institutional motivation in
engineering a space specific for this application are limited.  Thus, one
suggestion was to use third party testing of samples being submitted for
analysis.  However this is costly and most users have abandoned their
efforts to perform these measurements as a result.

 

I would be curious to know if third party testing of potentially infectious
materials such as human serum is commonly used within an EH&S setting, or if
it is possible to place a HEPA filter in the vacuum exhaust if the
instrument uses a TMP?

 

Thank you in advance for any information you may be able to offer.

 

Best regards

John Sumida

University of Washington.

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