[RASMB] Organic solvents in velocity runs

[Lake Paul]

John,

Additionally, what you have to worry about is the warping of the charcoal
Epon centerpieces with DMSO etc. When it comes to the DMSO, chloroform,
DMF, and toluene, I used the Al centerpieces (I know they are tedious, but
you don't have to worry about the organics interacting e.g. softening the
centerpieces). These have all been for SV experiments.

Lake

On Tue, Jan 16, 2018 at 3:34 PM, John Sumida <jpsumida at uw.edu> wrote:

> Dear RASMB,
>
>
>
> I’ve been reviewing some posts on the list regarding the use of various
> organic solvents in the AUC.  Most of the posts appear to refer
> specifically to equilibrium experiments as opposed to velocity experiments,
> some posts recommend the Kel-F centerpieces over the Charcoal-epon, some
> cite a small explosion hazard in the use of flammable organics.
>
>
>
> As there appears to be a wide variation in the posted recommendations I
> would like to ask two questions:
>
> 1.       Can the vacuum be exhausted into a fume hood in the case of an
> instrument with a turbo-molecular pump.
>
> 2.       Since many organic solvents are compressible (ie. chloroform,
> dichloromethane, toluene) and can thus generate a density difference in the
> cell, how does one account for these affects in a velocity experiment.
>
>
>
> As these questions are broad and I know your time is precious, any
> pertinent literature references that list members might point me at would
> be very helpful to me.  Additionally any operational/safety concerns list
> members might have compiled from experience working in non-aqueous solvents
> would also be appreciated.
>
>
>
> Best regards and thank you for your kind attention.
>
>
>
> John Sumida
>
> Molecular Analysis Facility
>
> University of Washington
>
>
>
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>
>
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