[RASMB] Optical alignment for AUC

[Laue, Thomas]

Dear RASMBers-
Will is entirely correct that the best way to align the cells is using the channel walls, which should fall along a radius. Spin Analytical has developed a commercial optical alignment tool that uses the same principle as outlined in the paper. Contact Kiara at Spin (kiara.jordon at spinanalytical.com<mailto:kiara.jordon at spinanalytical.com>) for more information.

I do want to point out that the Spin CAT tool is designed to work with Spin cell housings. The accuracy and precision of the cell alignment is impacted directly by the accuracy of the of the cell-housing slots used for alignment. Beckman housings have neither the requisite precision (housing-to-housing reproducibility) or accuracy (the slots must be perpendicular to the central cell radius). Hence, the added precision of Spin CAT alignment described in the paper is expected. Unfortunately, so is the reduced accuracy and precision from the Beckman cell housings.

Best wishes,
Tom


From: RASMB [mailto:rasmb-bounces at list.rasmb.org] On Behalf Of William F Weiss IV
Sent: Wednesday, May 24, 2017 8:39 AM
To: rasmb at list.rasmb.org
Subject: [RASMB] Optical alignment for AUC

Fellow RASMBers,
If you have ever struggled with alignment problems when using SV-AUC to quantify levels of soluble aggregates, then you might find this interesting:

An optical alignment system improves precision of soluble aggregate quantitation by sedimentation velocity analytical ultracentrifugation
ABSTRACT:
Appropriate characterization of soluble aggregates is an important aspect of biologics development and manufacturing, and sedimentation velocity analytical ultracentrifugation (SV-AUC) is often used an orthogonal technique to size-exclusion chromatography (SEC) for this purpose. Precise quantification of low levels of soluble aggregates by SV-AUC can be adversely impacted by improper cell alignment. This report describes the development of an optical system capable of quantifying cell alignment that affords a substantial improvement compared to historical approaches.

The journal provides temporary free access to the article:

https://authors.elsevier.com/a/1V5iolbALfkA<https://urldefense.proofpoint.com/v2/url?u=https-3A__authors.elsevier.com_a_1V5iolbALfkA&d=DwMFAg&c=c6MrceVCY5m5A_KAUkrdoA&r=hAcbVT23dt1uq6AGxObZKg&m=Kk2luH-2oREpqPNOyNI6EEokysKIf0nWeyqvnFM5Pj4&s=Qj5_pRSrgJeBHb6pT4zaNLDQSS4R_RCEQRyhiXFbSa8&e=>

We look forward to future developments in the field. Thanks.
-Will


William F. Weiss IV, Ph.D.
Principal Research Scientist
Biophysical Characterization Group Leader
Biopharmaceutical Research and Development
Eli Lilly and Company
317.433.8260 (office) | 317.209.4748 (mobile)
weisswf at lilly.com<mailto:weisswf at lilly.com> | www.lilly.com<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.lilly.com&d=DwMFAg&c=c6MrceVCY5m5A_KAUkrdoA&r=hAcbVT23dt1uq6AGxObZKg&m=Kk2luH-2oREpqPNOyNI6EEokysKIf0nWeyqvnFM5Pj4&s=evHR2lgbz2cy8Nj_nZvLv_7kZzkewD7mZEKh54JHJGI&e=>

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