[RASMB] Optical alignment for AUC

[Sambit Kar]

Thanks Will.

Sambit.

On Wed, May 24, 2017 at 8:38 AM, William F Weiss IV <
weiss_william_f_iv at lilly.com> wrote:

> Fellow RASMBers,
>
> If you have ever struggled with alignment problems when using SV-AUC to
> quantify levels of soluble aggregates, then you might find this interesting:
>
>
>
> *An optical alignment system improves precision of soluble aggregate
> quantitation by sedimentation velocity analytical ultracentrifugation*
>
> ABSTRACT:
>
> Appropriate characterization of soluble aggregates is an important aspect
> of biologics development and manufacturing, and sedimentation velocity
> analytical ultracentrifugation (SV-AUC) is often used an orthogonal
> technique to size-exclusion chromatography (SEC) for this purpose. Precise
> quantification of low levels of soluble aggregates by SV-AUC can be
> adversely impacted by improper cell alignment. This report describes the
> development of an optical system capable of quantifying cell alignment that
> affords a substantial improvement compared to historical approaches.
>
>
>
> The journal provides temporary free access to the article:
>
> https://authors.elsevier.com/a/1V5iolbALfkA
>
>
>
> We look forward to future developments in the field. Thanks.
>
> -Will
>
>
>
>
>
> *William F. Weiss IV, Ph.D.*
>
> Principal Research Scientist
>
> Biophysical Characterization Group Leader
>
> Biopharmaceutical Research and Development
>
> *Eli Lilly and Company*
>
> 317.433.8260 <(317)%20433-8260> (office) | 317.209.4748 <(317)%20209-4748>
> (mobile)
>
> weisswf at lilly.com | www.lilly.com
>
>
>
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