[RASMB] Taylor Dispersion SPR instrument

[Huber, Sylwia]

Dear John,

I am advanced user of SPR technology and we work mainly with small
molecules and DMSO solutions.
Such instrument as a SensiQ may surely work in high throughput mode.
I am, however, not very convinced of the benefit of concentration gradient
in a single injection.
We did some evaluation of SensiQ instrument and I think that after
concentration
gradient in a single injection, you have to make so or so an additional
experiment to verify the results.
Some of the data we have collected during the evaluation were not properly
evaluated by the software.

Basically, after injecting of a single concentration in a standard SPR
experiment, you get also the feeling where the affinity is. So my opinion
is, that the benefit of the single injection is not so high as promised and
we have decided not to invest in a such instrument. I hope to help you, but
please keep in mind it is my personal opinion.

Kind regards,
Sylwia



Mit freundlichen Grüssen/With Kind Regards

*Dr. Sylwia Huber*
Principal Scientist, *Biophysics Team Head*
Roche Innovation Center Basel
F. Hoffmann-La Roche Ltd., Chemical Biology
Building 65/203, Grenzacherstrasse 124
CH-4070 Basel, Switzerland
Phone: +41/61/6879507


On Wed, Mar 1, 2017 at 10:25 PM, John Sumida <jpsumida at uw.edu> wrote:

> Dear RASMB,
>
>
>
> This is not a question related to AUC.
>
>
>
> I am evaluating an instrument that uses a dispersion cell to inject a
> concentration gradient in a single injection to perform an SPR experiment.
> This would be similar to the SensiQ instrument that is no longer on the
> market.  I would love to get a sense of the utility of operating an
> instrument such as this in core lab environment.  I am particularly
> interested in assessing if this is truly a high throughput approach and how
> robust the approach is with respected to small molecules which may need
> DMSO in solution for solubility.
>
>
>
> Thank you for your time and comments in advance
>
>
>
> Best regards,
>
> John Sumida
>
> Molecular Analysis Facility
>
> University of Washington
>
>
>
> _______________________________________________
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> RASMB at list.rasmb.org
> http://list.rasmb.org/listinfo.cgi/rasmb-rasmb.org
>
>
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