[RASMB] Volatile buffers in AUC question

[David Hayes]

Cool:  a real explosion from an eye witness.  Thanks for the information Tom.Maybe Beckman is not being as over cautious as I originally thought. 

David

      From: "Laue, Thomas" <Tom.Laue at unh.edu>
 To: David Hayes <drdavidbhayes at yahoo.com>; "jphilo at mailway.com" <jphilo at mailway.com>; RASMB <rasmb at rasmb.org> 
 Sent: Thursday, October 15, 2015 10:03 AM
 Subject: RE: [RASMB] Volatile buffers in AUC question
   
#yiv7823142859 #yiv7823142859 -- _filtered #yiv7823142859 {font-family:Helvetica;panose-1:2 11 6 4 2 2 2 2 2 4;} _filtered #yiv7823142859 {font-family:Helvetica;panose-1:2 11 6 4 2 2 2 2 2 4;} _filtered #yiv7823142859 {font-family:Calibri;panose-1:2 15 5 2 2 2 4 3 2 4;} _filtered #yiv7823142859 {font-family:Tahoma;panose-1:2 11 6 4 3 5 4 4 2 4;}#yiv7823142859 #yiv7823142859 p.yiv7823142859MsoNormal, #yiv7823142859 li.yiv7823142859MsoNormal, #yiv7823142859 div.yiv7823142859MsoNormal {margin:0in;margin-bottom:.0001pt;font-size:12.0pt;}#yiv7823142859 a:link, #yiv7823142859 span.yiv7823142859MsoHyperlink {color:blue;text-decoration:underline;}#yiv7823142859 a:visited, #yiv7823142859 span.yiv7823142859MsoHyperlinkFollowed {color:purple;text-decoration:underline;}#yiv7823142859 p.yiv7823142859MsoAcetate, #yiv7823142859 li.yiv7823142859MsoAcetate, #yiv7823142859 div.yiv7823142859MsoAcetate {margin:0in;margin-bottom:.0001pt;font-size:8.0pt;}#yiv7823142859 p.yiv7823142859msonormal, #yiv7823142859 li.yiv7823142859msonormal, #yiv7823142859 div.yiv7823142859msonormal {margin-right:0in;margin-left:0in;font-size:12.0pt;}#yiv7823142859 p.yiv7823142859msochpdefault, #yiv7823142859 li.yiv7823142859msochpdefault, #yiv7823142859 div.yiv7823142859msochpdefault {margin-right:0in;margin-left:0in;font-size:12.0pt;}#yiv7823142859 span.yiv7823142859msohyperlink {}#yiv7823142859 span.yiv7823142859msohyperlinkfollowed {}#yiv7823142859 span.yiv7823142859emailstyle17 {}#yiv7823142859 p.yiv7823142859msonormal1, #yiv7823142859 li.yiv7823142859msonormal1, #yiv7823142859 div.yiv7823142859msonormal1 {margin:0in;margin-bottom:.0001pt;font-size:12.0pt;}#yiv7823142859 span.yiv7823142859msohyperlink1 {color:#0563C1;text-decoration:underline;}#yiv7823142859 span.yiv7823142859msohyperlinkfollowed1 {color:#954F72;text-decoration:underline;}#yiv7823142859 span.yiv7823142859emailstyle171 {color:blue;font-weight:normal;font-style:normal;text-decoration:none none;}#yiv7823142859 p.yiv7823142859msochpdefault1, #yiv7823142859 li.yiv7823142859msochpdefault1, #yiv7823142859 div.yiv7823142859msochpdefault1 {margin-right:0in;margin-left:0in;font-size:10.0pt;}#yiv7823142859 span.yiv7823142859EmailStyle27 {color:#1F497D;}#yiv7823142859 span.yiv7823142859BalloonTextChar {}#yiv7823142859 .yiv7823142859MsoChpDefault {font-size:10.0pt;} _filtered #yiv7823142859 {margin:1.0in 1.0in 1.0in 1.0in;}#yiv7823142859 div.yiv7823142859WordSection1 {}#yiv7823142859 Hi- The small amount and relatively low flammability of IPA (I thought you might be running an India Pale Ale) insures that there is no danger. Flammability is something to consider, however.   True story- we were working on the interference optics many years ago and had used some red glyptal to seal some holes in the heat sink. I was in a hurry to get it to dry, so turned on the pumps. Now glyptal has several very volatile and flammable solvents (mek, acetone, toluene), and, after a couple of minutes there was a pretty good sized explosion when a relay was thrown and the spark set off the gases. Thank god no one was hurt by my stupidity, there was no fire, and the only damage was a blown fuse. Best wishes, Tom    

From: RASMB [mailto:rasmb-bounces at list.rasmb.org]On Behalf Of David Hayes
Sent: Wednesday, October 14, 2015 1:46 PM
To: jphilo at mailway.com; RASMB
Subject: Re: [RASMB] Volatile buffers in AUC question    Hi John,    I was told that the outlets of the vacuum pumps went right into the inside of the centrifuge:  so the leak from the cell would be expelled as a vapor into the chassis of the centrifuge. Similarly, if a botox sample cell leaked, the toxin could go just about anywhere and they would have to throw away the centrifuge:  (a second hand apocryphal story from Allergan).    Kind Regards,    David          From: John Philo <jphilo at mailway.com>
To: 'David Hayes' <drdavidbhayes at yahoo.com>; 'RASMB' <rasmb at rasmb.org>
Sent: Wednesday, October 14, 2015 1:21 PM
Subject: RE: [RASMB] Volatile buffers in AUC question    David, if it were leaking into vacuum (no oxygen), how could it burn? Am I missing something?   John      From: RASMB [mailto:rasmb-bounces at list.rasmb.org]On Behalf Of David Hayes
Sent: Wednesday, October 14, 2015 9:59 AM
To: RASMB <rasmb at rasmb.org>
Subject: [RASMB] Volatile buffers in AUC question   Hi all,   I have a sample where we want to run in a buffer with 10% IPA. In general, because of the possibility of ignition of flammable components if there was a cell leak, People at AUC workshops say not to run organic solvents in the Beckman XLA/XLI instruments. Should I be worried about this buffer with 10% isopropyl alcohol?  Even if a cell leaked and 40 micro liters of IPA evaporated along with the aqueous components, how big of a flash would it be?  Could it damage the instrument?   I know that polymer chemists also run AUC and wonder if there is any actual experience with organic solvents and any verified incidents of cells leaking and causing fire damage.   Kind Regards,   David Hayes    

  
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