[RASMB] Volatile buffers in AUC question

[John Philo]

David, if it were leaking into vacuum (no oxygen), how could it burn? Am I missing something?

 

John

 

From: RASMB [mailto:rasmb-bounces at list.rasmb.org] On Behalf Of David Hayes
Sent: Wednesday, October 14, 2015 9:59 AM
To: RASMB <rasmb at rasmb.org>
Subject: [RASMB] Volatile buffers in AUC question

 

Hi all,

 

I have a sample where we want to run in a buffer with 10% IPA.

In general, because of the possibility of ignition of flammable components if there was a cell leak, People at AUC workshops say not to run organic solvents in the Beckman XLA/XLI instruments.

Should I be worried about this buffer with 10% isopropyl alcohol?  

Even if a cell leaked and 40 micro liters of IPA evaporated along with the aqueous components, how big of a flash would it be?  Could it damage the instrument?

 

I know that polymer chemists also run AUC and wonder if there is any actual experience with organic solvents and any verified incidents of cells leaking and causing fire damage.

 

Kind Regards,

 

David Hayes

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