[RASMB] XLI analysis of protein suspensions

[Prasad Satyamurthy]

Dear All,
            I am analysing a suspension of protein molecule. This suspension has crystals and soluble form of the same protein. To analyze this suspension and get information about the crystal size and stoke's radius of the soluble form of the protein I am running this sample in a sedimentation velocity experiment.
            In order to sediment the larger crystals I initially started with 6000 rpm later increased it to 55000 rpm. All the data was acquired using the interference detection system and was performed using the aluminium centre pieces with sapphire windows.
            So I would like to know if this is the right way of analysing such type of samples. Also which model or fitting program would be appropriate for such analysis?


Thanks and Regards



Prasad Satyamurthy





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All the work done with respect to this experiement are my personal experiemntal work done using the Analytical ultracentrifuge and bears no relation with the company.

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