[RASMB] SPR query about CFCA applications

[Markova, Natalia (GE Healthcare)]

Hi John,

We use - as default - f/f0 factor for globular proteins, which is 1.2 (not 1.5). However, user can freely enter any value for this factor in "Web diffusion coefficient calculator" (https://www.biacore.com/lifesciences/Application_Support/online_support/Diffusion_Coefficient_Calculator/index.html?section=lifesciences&realsection=lifesciences)
You need to be registered on the Biacore website to access it.

Best regards,

Natalia

Natalia Markova (PhD)
GE Healthcare
R&D Life Sciences
Rapsgatan 23
751 84 Uppsala
Sweden

M  +46 727 33 58 80
E   natalia.markova at ge.com<mailto:natalia.markova at ge.com>

www.biacore.com<https://exchange1.ad.slu.se/owa/redir.aspx?C=61606803db87492ba25280057e5d208e&URL=http%3a%2f%2fwww.biacore.com%2f>
www.microcal.com<https://exchange1.ad.slu.se/owa/redir.aspx?C=61606803db87492ba25280057e5d208e&URL=http%3a%2f%2fwww.microcal.com%2f>


Disclaimer
The information contained in this e-mail is intended solely for the use of the addressee and contains information that is confidential and may be legally privileged. Access to this e-mail by anyone else is unauthorised. If you are not the intended recipient, your use, retention, copy, any disclosure, dissemination or any action taken or omitted to be taken in reliance of this communication is strictly prohibited and may be unlawful. If you have received this communication in error, please notify us immediately by phone. Thank you!




From: rasmb-bounces at list.rasmb.org [mailto:rasmb-bounces at list.rasmb.org] On Behalf Of John Sumida
Sent: den 14 januari 2014 00:20
To: rasmb at rasmb.org
Subject: [RASMB] SPR query about CFCA applications

Dear RASMB,

This question is in regards to an SPR application.  We are currently investigating the use "calibration free concentration analysis" (CFCA) to monitor the stability of macromolecular preps over time in terms of their activity.  Input parameters for this analysis are ff0, viscosity, MW and D and we are using AUC to better determine both.  Current applications seem to assume ff0=1.5, which may not be correct for monoclonal preps based on my reading of the literature.

My query.

1.       Is it currently the common practice to measure these quantities and if so what is the preferred method to do so?

Thank you all and Happy New Year,

John Sumida, Ph.D.
Analytical Biopharmacy Core Facility<http://depts.washington.edu/cidb4bio/index.shtml>
University of Washington
Molecular Engineering & Sciences Institute, G22<http://www.moles.washington.edu/>
3946 West Stevens Way NE
Seattle WA 98195-1653

-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://list.rasmb.org/pipermail/rasmb-rasmb.org/attachments/20140114/cb5d5a42/attachment.htm>