[RASMB] Viscometer

[Razinkov, Vladimir]

Dear all,

Another rather simple and high throughput  method to measure viscosity of proteins at low volumes (~ 35 uL with 384-well plate) using DLS plate reader.

Anal Biochem.<http://www.ncbi.nlm.nih.gov/pubmed/19995543> 2010 Apr 1;399(1):141-3. doi: 10.1016/j.ab.2009.12.003. Epub 2009 Dec 6.
High-throughput dynamic light scattering method for measuring viscosity of concentrated protein solutions.
He F<http://www.ncbi.nlm.nih.gov/pubmed?term=He%20F%5BAuthor%5D&cauthor=true&cauthor_uid=19995543>, Becker GW<http://www.ncbi.nlm.nih.gov/pubmed?term=Becker%20GW%5BAuthor%5D&cauthor=true&cauthor_uid=19995543>, Litowski JR<http://www.ncbi.nlm.nih.gov/pubmed?term=Litowski%20JR%5BAuthor%5D&cauthor=true&cauthor_uid=19995543>, Narhi LO<http://www.ncbi.nlm.nih.gov/pubmed?term=Narhi%20LO%5BAuthor%5D&cauthor=true&cauthor_uid=19995543>, Brems DN<http://www.ncbi.nlm.nih.gov/pubmed?term=Brems%20DN%5BAuthor%5D&cauthor=true&cauthor_uid=19995543>, Razinkov VI<http://www.ncbi.nlm.nih.gov/pubmed?term=Razinkov%20VI%5BAuthor%5D&cauthor=true&cauthor_uid=19995543>.
Source
Formulation and Analytical Resources, Amgen Inc., Seattle, WA 98119, USA.
Abstract
We propose a new method to measure the viscosity of concentrated protein solutions in a high-throughput format. This method measures the apparent hydrodynamic radius of polystyrene beads with known sizes using a dynamic light scattering (DLS) system with a microplate reader. Glycerol solution viscosities obtained by the DLS method were in good agreement with those reported in the literature. Viscosity of the solutions of two monoclonal antibody molecules was acquired using both DLS and cone-and-plate techniques, and the results were comparable. The DLS method described here has the potential to be used in many aspects of protein characterization


Regards

Vladimir Razinkov

From: rasmb-bounces at list.rasmb.org [mailto:rasmb-bounces at list.rasmb.org] On Behalf Of Richard Kingston
Sent: Wednesday, June 05, 2013 9:15 PM
To: rasmb at rasmb.org
Subject: [RASMB] Viscometer


Dear all,

I've become interested in measuring the viscosity of protein solutions at varying temperature,  and wondered if anyone on the list could advise on the best way to do this using commercially available instrumentation. While there are many  ways to measure viscosity, for protein applications minimizing the required sample volume is pretty critical. I note that  Anton Paar sell an instrument that measures both dynamic viscosity and density  (SVM 3000 Stabinger viscometer). If you skip the density measurement, the sample requirements look almost "manageable". Cambridge also sell a micro-sample viscometer (Viscolab 5000) which uses microlitre volumes.

I'm having difficulty tracking down people that might have used these or similar instruments, and could comment on the practicalities for protein work. If anyone can offer advice, either on- or off-list, it would be appreciated.

Many thanks,

Richard

Richard Kingston, PhD.
School of Biological Sciences
The University of Auckland
New Zealand

website: http://persephone.sbs.auckland.ac.nz/richard/lab/



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