[RASMB] Amyloid Fibrils: AF4-MALS Characterization

[William Wittbold]

Hello all,
   In response to Sabine Kaltofen's inquiry:


Dear all,

has anyone of you tried to describe the size/molecular weight of amyloid fibrils using the Fujita approach?

I am after a rather accurate determination of the MW (distribution) of fibrils. Therefore, using a scaling law to relate s with M seems the most  rigorous thing to do. I am, however not aware of any literature where  this approach has been used for peptide/protein fibrils. Am I missing a major point here, why this is not applicable? All comments, experiences and thoughts are greatly appreciated!



Best wishes

Sabine



I have a collaboration to fractionate and characterize a-beta distribution in Alzheimer's afflicted brains vs. healthy brains via asymmetrical field flow fractionation coupled to multi-angle static light scattering. Our Boston application lab uses the Eclipse DualTec AF4 system together with an Agilent UV detector, and a Wyatt DAWN HELEOS-II MALS and Optilab T-rEX RI detector for this work, and our preliminary data show a Mw distribution of 5 kDa up to hundreds of MDa.  If you have specific questions, I'd be happy to answer them.



Best Regards,

Will


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William Wittbold | Application Scientist | Northeast Customer Service Manager
Wyatt Technology Corporation
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