[RASMB] Nanoparticle absorbance question

Deborah Pheasant pheasant at MIT.EDU
Wed Jun 13 12:10:10 PDT 2012


Hi all,
We had a suspension of nanoparticles (metal oxides) in culture medium with FBS proteins and it measured 0.5 AU at 530 nm in a UV/VIS spec.  When we put it into SV centerpieces, it read that it had an A530 of about -0.1 or -0.2.  We are perplexed as to why this should be.  Have you had similar experiences or do you have an idea about what might be going on?
Thanks very much,
Debby Pheasant
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