[RASMB] CPU Board Malfunction on XL-I

[Mark Agacan]

Hi,
 
I'm confused about which value to use in the calculation of dimerization and tetramerization constants in Sedphat.
 
e.g. monomer-tetramer-octamer self-association model:
 
logKa14 = 3.127, then Ka14 = 1339.677 M3, so 1/Ka14 gives 7.465 x 10-4, or 746.5 uM (which should be the equilibrium dissociation constant for the monomer : tetramer interaction).
 
But the thermo_results file says c1 = c4 at 9.069 x 10-2 M = 90.69 mM.  Is this then the tetramerization constant?
 
 
I also processed the same data using monomer-n-mer self association model with n = 4:
 
logKa1n = 3.047, then Ka1n = 1.114 x 10+3 M3, so 1/Ka1n = 4.487 x 10-4, or 448.7 uM.
 
Again the thermo_results file says c1 = c4 at 9.642 x 10-2 M = 96.42 mM.
 
Can someone explain please?
 
Many Thanks,

Mark
 
 
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Dr Mark Agacan, Scientific Officer for the Division of Biological Chemistry and Drug Discovery,
Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dundee, DD1 5EH 
Tel: +44 1382 386095    Fax: +44 1382 345764    Mobile: 07525 451 117
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