[RASMB] AUC capabilities

[Walter Stafford]

Hi Ewa,

We have been able to measure the dissociation constant of about 50 nM in 
the following system using interference optics, and global fitting a 
range of concentrations and component ratios with SEDANAL:

"The endoplasmic reticulum lumenal domain of the adenovirus type 2 
E3-19K protein binds to peptide-filled and peptide-deficient HLA-A*1101 
molecules."
Liu H, Stafford WF, Bouvier M. J Virol. 2005 Nov;79(21):13317-25.

PMID: 16227254 [PubMed - indexed for MEDLINE] Free PMC Article Free text

This is probably about as low as one could go (at this molar mass) using 
intereference optics and still have some confidence in the results.

As has already been pointed out by Borries, fluorescence optics would 
get you more into the range you need.

Walter

-- 

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Walter F. Stafford, Ph.D.
Senior Scientist
Boston Biomedical Research Institute
64 Grove Street
Watertown, MA 02472
617-658-7808
skype: w.stafford3
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"Be kind, for everyone you meet is fighting a hard battle."  -Plato
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