[RASMB] FW: sedimentation velocity data

[John Philo]

Anamika, it's a little hard to tell what is going on because the majority of
the scans are after the cell is completely empty (you don't want to load
those). There is however, at the very least, something funny about the
spacing of the scans over time (the apparent amount of boundary movement
between successive scans varies substantially), and that might indeed be due
to some problem with the slit motion or radial readout. I think I may be
seeing the apparent meniscus position shift back and forth randomly rather
than in a consistent direction over time (it's hard to tell with so many
scans overlapping), and if that is true then yes you have a serious hardware
problem and need to bring in the service technician (but don't describe the
problem to Beckman as a 'radial calibration' problem). If that is what is
happening you really won't be able to analyze the data.
 
As an aside, you really need to fill your cells much more completely. Try to
get the sample meniscus to the left of 6.00 cm (I recommend at least 420
microliters of sample).
 
John
  _____  

From: rasmb-bounces at rasmb.bbri.org [mailto:rasmb-bounces at rasmb.bbri.org] On
Behalf Of Anamika Patel
Sent: Friday, September 30, 2011 9:59 AM
To: RASMB at rasmb.bbri.org
Subject: [RASMB] FW: sedimentation velocity data


Sorry I missed the atatchement in my earlier e-mail
 
Anamika
 
  _____  

From: Anamika Patel
Sent: Friday, September 30, 2011 11:00 AM
To: RASMB at rasmb.bbri.org
Subject: sedimentation velocity data 


Dear RASMB subscribers,
 
We collected some sedimentation velocity data on AUC (XLA) and happed to
notice a problem with our mechine with radial cabritation. As a result of
that in our data the sample as well as refrence mesicuse drifted a lot from
scan to scan. It seems that we need some fixing with our AUC but meanwhile I
was wondering if there is any way to analyze the data which I have already
collected. I have atatched a snapshot of my data. Any suggestions or advice
would be highly appreciated.
 
Thanks
 
 
Anamika Patel, Ph.D.
Department of Biology
Life Science Complex
Syracuse University
107 College place
Syracuse- 13244
NY
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