[RASMB] Interference optics problem

[Leech, AP]

Hi Paul,

Do your fringe patterns look ok? I take it you have tried readjusting
the laser delay and duration? Perhaps if you could post up some of the
bad data it would help.

Best regards,

Andrew


On 27/09/2011 14:15, Paul Leonard wrote:
> Hi,
>
> I am hoping one of you may be able to help me. We have seen a significant
> drop in our interference optics detection signal and we are getting jumps
> in the data (as if shifting to a different baseline) at several places
> across the cell sample volume.
>
> We think that the problem could have arisen because the AUC was running
> during an earthquake. The earthquake was not that strong here but you
> certainly felt it and every AUC run since has generated unusable
> interference data.
>
> We are wondering if something has been knocked or become mis-aligned and
> were thinking that we could try readjusting the mirrors for the
> interference optics to see if we can increase the signal. However we
> haven't been able to find instructions in the manual for what exactly we
> should be looking for when we do this.
>
> Can anyone help?
>
> If you have any other suggestions for any other things that could have
> gone wrong and possible ways to diagnose and fix the problem I would be
> very grateful to hear them.
>
> best wishes
>
> Paul
>
>
> Department of Biochemistry
> UMDNJ-Robert Wood Johnson Medical School
> Center for Advanced Biotechnology and Medicine
> 679 Hoes Lane
> Piscataway
> New Jersey 08854-5627
>
> Phone: (732) 235-4206
> Email: leonard at cabm.rutgers.edu
>
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-- 
Dr Andrew Leech                   *  Laboratory Head
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