[RASMB] Intensity Scan Help

Borries Demeler demeler at biochem.uthscsa.edu
Sat Jul 23 06:24:18 PDT 2011


Peter,

I have summarized a few suggestions here:

http://www.uslims.uthscsa.edu/lamp_diagnostics.php

Your variation is about right, but it is usually a function
of noise in the optics, which could include oil on the 
slit assembly parts, and the monochromator.

Regards, -Borries
> 
> Hi All
> 
> We are still having problems with our XL-A.  I have run intensity scans acr=
> oss an empty rotor hole at 280  (counterbalances in pos 2 & 4, scan cell 3)=
> . I'm not sure I set it up right  but if I did the intensity averages 6700 =
> but varies from about 6500 to 7500. Apart from much lower noise fluctuation=
> s it appears that the reference and sample land on top of one another at al=
> l points.  What sort of variation should I be seeing with radial distance.
> 
> What sort of tests should I be doing to evaluate the optical system?
> 
> 
> Thanks
> 
> Peter
> 
> Peter E. Prevelige Jr.
> Professor
> Dept. of Microbiology, BBRB 416/6
> Univ. of Alabama @ Birmingham
> 845 19th St. South
> Birmingham AL. 35294-2170
> Phone 205 975-5327
> FAX 205 975-5479
> prevelig at uab.edu<mailto:prevelig at uab.edu>
> http://www.microbio.uab.edu/faculty/prevelige/prevelige-p.htm





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