[RASMB] centerpiece/window compatibility with decontaminatingsolution

[sumit goswami]

So decontamination of the centerpiece and windows with 10% bleach and
autoclaving the cell housing at 121 degree-C for 30min. Erby Perdue
mentioned that autoclaving cell housing for that much time will be okay.

Thanks to every one.

Sumit

On Fri, Apr 1, 2011 at 10:03 AM, John Philo <jphilo at mailway.com> wrote:

>  Sumit, the Beckman materials compatibility guide that comes with the
> centrifuge (also available on their web site) lists commercial bleach and
> says its use with epoxy (Epon) is satisfactory. That list doesn't have an
> entry for sapphire but I'm pretty sure it can stand up to bleach. Any
> detergent is okay with centerpieces and windows.
>
> I think your biggest decontamination issue is going to be the cell
> housing. During filling you almost inevitably get some sample solution
> coming out around the fill holes and hence into the threads of the housing.
> The aluminum housings definitely cannot stand up to bleach.
>
> John
>
>  ------------------------------
>  *From:* rasmb-bounces at rasmb.bbri.org [mailto:rasmb-bounces at rasmb.bbri.org]
> *On Behalf Of *sumit goswami
> *Sent:* Friday, April 01, 2011 7:27 AM
> *To:* Brandon L Doyle
> *Cc:* rasmb at rasmb-email.bbri.org; rasmb-bounces at rasmb.bbri.org
> *Subject:* Re: [RASMB] centerpiece/window compatibility with
> decontaminatingsolution
>
>  Hi,
> I guess I was wrong when I talked about using contrad for decontaminating
> cell assembly. Contrad is a detergent so most likely it's not gonna be that
> effective for decontamination. Bertrand mentioned about Vikron as
> decontaminating agent. I will try to buy it. At this point I have bleach
> solution. Does anybody know if that is okay with epoxy resin and sapphire?
>
> Thank you so much!
> Sumit
>
> On Fri, Apr 1, 2011 at 7:51 AM, Brandon L Doyle <DOYLE_BRANDON_L at lilly.com
> > wrote:
>
>>
>> Sumit,
>>
>>  I am not sure what variety of Contrad or concentration you are planning
>> on using for decontamination, but we routinely use Contrad70 (about 0.1-1%
>> vol/vol solutions) on Epon cells with no apparent ill effects.  We have not
>> noticed any etching of windows (or quartz spectroscopy cells for that
>> matter) with those dilute conditions either.
>>
>> Brandon
>>
>>
>>
>>
>>   *sumit goswami <ikant11 at gmail.com>*
>> Sent by: rasmb-bounces at rasmb.bbri.org
>>
>> 03/31/2011 10:19 PM
>>    To
>> rasmb at rasmb-email.bbri.org
>> cc
>>   Subject
>> [RASMB] centerpiece/window compatibility with decontaminating
>>  solution
>>
>>
>>
>>
>> Hello All,
>>              I am planning to run some sed-vel experiments with a protein
>> that is cytotoxic. That means the components of the cell assembly will
>> require decontamination followed by the experiment. Can anyone tell me if
>> the centerpieces (Epon charcoal-filled) and the sapphire windows are going
>> to be okay with bleach or contrad? I am more concerned about the
>> centerpieces.
>>
>> Thank you so much!
>> With regards,
>> Sumit_______________________________________________
>>
>> RASMB mailing list
>> RASMB at rasmb.bbri.org
>> http://rasmb.bbri.org/cgi-bin/mailman/listinfo/rasmb
>>
>>
>
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