[RASMB] centerpiece/window compatibility with decontaminatingsolution

[John Philo]

Sumit, the Beckman materials compatibility guide that comes with the
centrifuge (also available on their web site) lists commercial bleach and
says its use with epoxy (Epon) is satisfactory. That list doesn't have an
entry for sapphire but I'm pretty sure it can stand up to bleach. Any
detergent is okay with centerpieces and windows.
 
I think your biggest decontamination issue is going to be the cell housing.
During filling you almost inevitably get some sample solution coming out
around the fill holes and hence into the threads of the housing. The
aluminum housings definitely cannot stand up to bleach. 
 
John

  _____  

From: rasmb-bounces at rasmb.bbri.org [mailto:rasmb-bounces at rasmb.bbri.org] On
Behalf Of sumit goswami
Sent: Friday, April 01, 2011 7:27 AM
To: Brandon L Doyle
Cc: rasmb at rasmb-email.bbri.org; rasmb-bounces at rasmb.bbri.org
Subject: Re: [RASMB] centerpiece/window compatibility with
decontaminatingsolution


Hi, 
I guess I was wrong when I talked about using contrad for decontaminating
cell assembly. Contrad is a detergent so most likely it's not gonna be that
effective for decontamination. Bertrand mentioned about Vikron as
decontaminating agent. I will try to buy it. At this point I have bleach
solution. Does anybody know if that is okay with epoxy resin and sapphire? 
 
Thank you so much!
Sumit


On Fri, Apr 1, 2011 at 7:51 AM, Brandon L Doyle <DOYLE_BRANDON_L at lilly.com>
wrote:



Sumit, 

 I am not sure what variety of Contrad or concentration you are planning on
using for decontamination, but we routinely use Contrad70 (about 0.1-1%
vol/vol solutions) on Epon cells with no apparent ill effects.  We have not
noticed any etching of windows (or quartz spectroscopy cells for that
matter) with those dilute conditions either. 

Brandon 





sumit goswami <ikant11 at gmail.com> 
Sent by: rasmb-bounces at rasmb.bbri.org 


03/31/2011 10:19 PM 


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Subject
[RASMB] centerpiece/window compatibility with decontaminating
solution	

		





Hello All, 
             I am planning to run some sed-vel experiments with a protein
that is cytotoxic. That means the components of the cell assembly will
require decontamination followed by the experiment. Can anyone tell me if
the centerpieces (Epon charcoal-filled) and the sapphire windows are going
to be okay with bleach or contrad? I am more concerned about the
centerpieces. 
  
Thank you so much! 
With regards, 

Sumit_______________________________________________ 

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