[RASMB] V-bar calculation for mAb

[sumit goswami]

Hello All,
             I did what Jhon suggested (HC & LC sequence merged, disulfide
linkage allowed and selected N=2 for dimer) and found out the v-bar was
0.7305. Thank you so much to all of you!

With regards,
Sumit

On Wed, Mar 23, 2011 at 7:44 AM, Mattia Rocco <mattia.rocco at istge.it> wrote:

>
> Dear Sumit,
>
> I second what John Philo said. In addition, we have available a program,
> PROMOLP, which among other things computes the vbar from the sequence,
> taking into account carbohydrates and phosphates. You can manually enter a
> sequence, or import it from a SwissProt-formatted file. I'll be glad to send
> it to you, or to anyone else who might want it, upon request.
>
> Best - Mattia
>
>
> At 02:21 PM 3/22/2011 -0500, sumit goswami wrote:
>
> Hello all,
>              I was trying to calculate v-bar of a mAb using SEDNTERP. I
> have the sequence of heavy chain (HC) and light chain (LC) seperately as
> expected. It seems to be little complicated. Because there are HC-HC and
> LC-LC disulfide linkages too.The question is how do I deal with that? Should
> I calculate the v-bar of Fab and Fc seperately and then calculate an weight
> avergae of them?
>
> With regards,
> Sumit
> _______________________________________________
> RASMB mailing list
> RASMB at rasmb.bbri.org
> http://rasmb.bbri.org/cgi-bin/mailman/listinfo/rasmb
>
>
> Dr. Mattia Rocco
> Biopolimeri e Proteomica
> Istituto Nazionale per la Ricerca sul Cancro (IST)
> c/o Centro per le Biotecnologie Avanzate (CBA)
> IST c/o CBA, Largo Rosanna Benzi 10
> I-16132 Genova, Italy
>
> Phone: +39-0105737-310
> Fax: +39-0105737-325
> e-mail: mattia.rocco at istge.it
>
> ATTENZIONE.
> Il presente messaggio ed i suoi allegati devono intendersi ad uso esclusivo
> dei suoi destinatari e sono confidenziali. Persone diverse dal destinatario,
> anche ai sensi del D.Lgs. n. 196/2003 , non debbono prendere visione dei
> contenuti, copiare od inoltrare l'e-mail a terzi. Se ricevete questo
> messaggio per errore, Vi preghiamo di cancellarlo, di distruggerne ogni
> copia e di informarci immediatamente. La mail non è un protocollo sicuro,
> pertanto IST declina ogni responsabilità in caso di intercettazione o
> modifiche del presente messaggio.
> *WARNING.
> This message and any attachments is intended solely for the use of the
> intended
> addressees and is confidential. Any unauthorized review, use, disclosure or
> distribution
> is prohibited. If you receive this message in error, please delete it,
> destroy all copies
> and immediately notify us. Mail is not a secure protocol, therefore IST
> will not be liable
> for interception or amendment of this message.
> *
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://list.rasmb.org/pipermail/rasmb-rasmb.org/attachments/20110323/36a60fe3/attachment.htm>