[RASMB] V-bar calculation for mAb

[Mattia Rocco]

Dear Sumit,

I second what John Philo said. In addition, we have available a program, 
PROMOLP, which among other things computes the vbar from the sequence, 
taking into account carbohydrates and phosphates. You can manually enter a 
sequence, or import it from a SwissProt-formatted file. I'll be glad to 
send it to you, or to anyone else who might want it, upon request.

Best - Mattia

At 02:21 PM 3/22/2011 -0500, sumit goswami wrote:
>Hello all,
>              I was trying to calculate v-bar of a mAb using SEDNTERP. I 
> have the sequence of heavy chain (HC) and light chain (LC) seperately as 
> expected. It seems to be little complicated. Because there are HC-HC and 
> LC-LC disulfide linkages too.The question is how do I deal with that? 
> Should I calculate the v-bar of Fab and Fc seperately and then calculate 
> an weight avergae of them?
>
>With regards,
>Sumit
>_______________________________________________
>RASMB mailing list
>RASMB at rasmb.bbri.org
>http://rasmb.bbri.org/cgi-bin/mailman/listinfo/rasmb


Dr. Mattia Rocco
Biopolimeri e Proteomica
Istituto Nazionale per la Ricerca sul Cancro (IST)
c/o Centro per le Biotecnologie Avanzate (CBA)
IST c/o CBA, Largo Rosanna Benzi 10
I-16132 Genova, Italy

Phone: +39-0105737-310
Fax: +39-0105737-325
e-mail: mattia.rocco at istge.it

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