[RASMB] noisy interference data

[Tom Laue]

Hi Mark-
The interference system requires the use of sapphire windows, except at 
very low rotor speeds.
Modern synthetic sapphire windows may be used to 230 nm. The old 
sapphire windows were not clear much past 280 nm, but that is no longer 
an issue.
I know that some labs have switched to using only sapphire window since 
they almost never break, and may be used with all three optical systems.
Best wishes,
Tom

On 2/24/2011 4:37 AM, Mark Agacan wrote:
> Hello,
> I have attached a jpeg image showing recent SV interference data plots 
> taken from sedfit.
> All data was collected with quartz windows as I was primarily 
> interested in the absorbance @ 280 nm signal.
> IP2 and IP6 are very noisy but IP4 looks fine.
> Is this a calibration problem or is this related to damaged windows / 
> centerpieces (there are no visible defects or scratches on any of the 
> components).
> Many Thanks,
> Mark
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> Dr Mark Agacan, Scientific Officer for the Division of Biological 
> Chemistry and Drug Discovery,
> Wellcome Trust Biocentre, College of Life Sciences, University of 
> Dundee, Dundee, DD1 5EH
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Department of Biochemistry and Molecular Biology
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