[RASMB] noisy interference data

[Mark Agacan]

I checked the laser settings as advised and found:
 
 


cell
laser delay
laser duration
exposure time
2157.1 deg

1.7 deg6.30 uS
4
67.1 deg
0.6 deg
2.22 uS
6
337.6 deg
1.7 deg
6.3 uS
 
I also found this in the rasmb archives:
 
"The main "gotcha" about setting the duration is that if you go too high you
will end up with the superposition of a single-slit interference pattern upon the
double-slit interference pattern.  As long as you use the typical 0.5 - 0.8
degrees for the duration (assuming that you are NOT using the old style interference
window holders) you will be fine."
 
So  I'm thinking to bring the laser duration for cell2 and cell6 down to the value of cell4.  Any further advice is greatly appreciated.
 
Many Thanks,
 
Mark
 
 
 
 
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Mark Agacan, Scientific Officer for the Division of Biological Chemistry and Drug Discovery,
Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dundee, DD1 5EH 
Tel: +44 1382 386095    Fax: +44 1382 345764    Mobile: 07525 451 117
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 
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>>> Tom Laue <Tom.Laue at unh.edu> 2/24/2011 13:05 >>>
Hi Mark-
The interference system requires the use of sapphire windows, except at 
very low rotor speeds.
Modern synthetic sapphire windows may be used to 230 nm. The old 
sapphire windows were not clear much past 280 nm, but that is no longer 
an issue.
I know that some labs have switched to using only sapphire window since 
they almost never break, and may be used with all three optical systems.
Best wishes,
Tom

On 2/24/2011 4:37 AM, Mark Agacan wrote:
> Hello,
> I have attached a jpeg image showing recent SV interference data plots 
> taken from sedfit.
> All data was collected with quartz windows as I was primarily 
> interested in the absorbance @ 280 nm signal.
> IP2 and IP6 are very noisy but IP4 looks fine.
> Is this a calibration problem or is this related to damaged windows / 
> centerpieces (there are no visible defects or scratches on any of the 
> components).
> Many Thanks,
> Mark
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> Dr Mark Agacan, Scientific Officer for the Division of Biological 
> Chemistry and Drug Discovery,
> Wellcome Trust Biocentre, College of Life Sciences, University of 
> Dundee, Dundee, DD1 5EH
> Tel: +44 1382 386095    Fax: +44 1382 345764    Mobile: 07525 451 117
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> ************************************************************
> Please consider the environment. Do you really need to print this email?
>
> The University of Dundee is a registered Scottish charity, No: SC015096
>
>
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-- 
Department of Biochemistry and Molecular Biology
University of New Hampshire
Durham, NH 03824-3544
Phone: 603-862-2459
FAX:   603-862-0031
E-mail: Tom.Laue at unh.edu
www.bitc.unh.edu
www.camis.unh.edu


The University of Dundee is a registered Scottish charity, No: SC015096
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