[RASMB] AUC capabilities

[Ewa Folta-Stogniew]

Hi Walter,

thanks.  I have no access to FL optics at Yale, but will consider 
Allen's approach (if I can locate all the hardware and the amount of 
protein needed is not prohibitive).

Can you give me an assessment of how much protein was used for this study?

Ewa

At 10:47 AM 1/27/2011, Walter Stafford wrote:
>Hi Ewa,
>
>We have been able to measure the dissociation constant of about 50 
>nM in the following system using interference optics, and global 
>fitting a range of concentrations and component ratios with SEDANAL:
>
>"The endoplasmic reticulum lumenal domain of the adenovirus type 2 
>E3-19K protein binds to peptide-filled and peptide-deficient 
>HLA-A*1101 molecules."
>Liu H, Stafford WF, Bouvier M. J Virol. 2005 Nov;79(21):13317-25.
>
>PMID: 16227254 [PubMed - indexed for MEDLINE] Free PMC Article Free text
>
>This is probably about as low as one could go (at this molar mass) 
>using intereference optics and still have some confidence in the results.
>
>As has already been pointed out by Borries, fluorescence optics 
>would get you more into the range you need.
>
>Walter
>
>--
>
>===============================================
>Walter F. Stafford, Ph.D.
>Senior Scientist
>Boston Biomedical Research Institute
>64 Grove Street
>Watertown, MA 02472
>617-658-7808
>skype: w.stafford3
>----------------------
>"Be kind, for everyone you meet is fighting a hard battle."  -Plato
>------------------------------------