[RASMB] AUC capabilities

[Nikola Wenta]

Hi Ewa!
I have successfully extracted the dimerization constant for my 90 kDa protein by SE (Kd 50 nM), using ABS optics in the range of 220-230 nm and multiple speeds and concentrations. The data was analysed globally with the very powerful and easy-to-use Ultrascan software ( http://ultrascan.uthscsa.edu , available for Windows, Linux and Mac). Most important is that you try to cover a concentration range as broad as possible, using serial protein dilutions with apropriate wavelengths. Make use of an 8-hole rotor and 6-channel centerpieces and several speeds; Ultrascan can simulate sedimentation gradients and give you reliable estimates for the time required until your protein is in equilibrium, and you can directly put the speed/time program into the data acquisition software. You can find my paper in Pubmed: Wenta et al., PNAS (2008). Don't hesitate to ask if you have further questions.
Niko
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