[RASMB] RASMB Digest, Vol 16, Issue 1
Virgil Schirf
schirfv at biochem.uthscsa.edu
Fri Jan 7 09:55:00 PST 2011
Hi Xin,
The counterbalance is used to calibrate the AUC radially. There are
masks, held in place by set screws, inside the counterbalance which have
an edge of 5.85 cm on the inside and 7.15 cm on the outside. You should
also notice that there is an offset of the mask on the inside edge
between the 'reference' side and the 'sample side'. The counterbalance
is placed in hole 4 of the An60 Ti or in hole 8 of An50 Ti rotor. You
will need to perform radial calibrations periodically if you are using
the same rotor and every time you change rotors/counterbalances.
You can go to http://cauma.uthscsa.edu and under the Home Links is an
AUC Hardware User Guide put together by Dr. Karel Planken and myself
where there is more of a description of the counterbalance and other
hardware information about the AUC.
Best regards,
Virgil
Xin-Ping Xu/FS/VCU wrote:
> Hi, everyone,
> I am also a beginner in AUC, could someone tell me what are the
> functions of counter balance? If it is just as a balance, we can use
> other cell as balance.
> Thanks.
> Xin Xu
>
>
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> Subject: RASMB Digest, Vol 16, Issue 1
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> Today's Topics:
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> 1. question about counter balance (Jia Bei Lin)
> 2. Re: question about counter balance (Walter Stafford)
> 3. Re: question about counter balance (Leech, AP)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 6 Jan 2011 17:29:37 -0600
> From: Jia Bei Lin <jiabei at uab.edu>
> To: "rasmb at rasmb.bbri.org" <rasmb at rasmb.bbri.org>
> Subject: [RASMB] question about counter balance
> Message-ID:
> <2A5095E179BC5F4F87BF7B8DBAEBD320333AA6B752 at UABEXMBS1.ad.uab.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> Dear professors and colleagues,
>
> I'm in the learning process of using the Beckman XL-A analytical ultracentrifuge. There are two counter balances in the lab, one of them is 33.6 grams and the other is 31.7 g just by themselves (no weight inside). Why are they made in different weight? They look identical from outside, what's inside made the difference?
>
>
> Thank you very much. Happy new year.
>
> Jiabei Lin
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> ------------------------------
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> Message: 2
> Date: Thu, 06 Jan 2011 18:45:21 -0500
> From: Walter Stafford <stafford at bbri.org>
> To: Jia Bei Lin <jiabei at uab.edu>
> Cc: "rasmb at rasmb.bbri.org" <rasmb at rasmb.bbri.org>
> Subject: Re: [RASMB] question about counter balance
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> Message: 3
> Date: Fri, 07 Jan 2011 10:55:20 +0000
> From: "Leech, AP" <apl3 at york.ac.uk>
> To: Jia Bei Lin <jiabei at uab.edu>
> Cc: "rasmb at rasmb.bbri.org" <rasmb at rasmb.bbri.org>
> Subject: Re: [RASMB] question about counter balance
> Message-ID: <4D26F118.40102 at york.ac.uk>
> Content-Type: text/plain; charset=UTF-8; format=flowed
>
> Hi Jia Bei, Walt,
>
> We have two centrepieces, one weighs 31.62 g and the other 31.91 g
> without weights.
>
> The weights themselves are also not quite exact, they vary up to
> about 0.05 g from the nominal marked weight.
>
> Andrew
>
> On 06/01/2011 23:45, Walter Stafford wrote:
> > Hi Jiabei;
> >
> > It's possible that one of them is missing the masks (held in by a small
> > hex screw) that provide the reference edges for the radial calibration.
> > Take a close look. It may not be obvious at first glance.
> >
> > Walter
> >
> > --
> >
> > ===============================================
> > Walter F. Stafford, Ph.D.
> > Senior Scientist
> > Boston Biomedical Research Institute
> > 64 Grove Street
> > Watertown, MA 02472
> > 617-658-7808
> > skype: w.stafford3
> > ----------------------
> > "Be kind, for everyone you meet is fighting a hard battle." -Plato
> > ------------------------------------
> >
> >
> >
> > Jia Bei Lin wrote:
> >>
> >> Dear professors and colleagues,
> >>
> >> I?m in the learning process of using the Beckman XL-A
> >> analytical ultracentrifuge. There are two counter balances in the lab,
> >> one of them is 33.6 grams and the other is 31.7 g just by themselves
> >> (no weight inside). Why are they made in different weight? They look
> >> identical from outside, what?s inside made the difference?
> >>
> >> Thank you very much. Happy new year.
> >>
> >> Jiabei Lin
> >>
> >>
> >> _______________________________________________
> >> RASMB mailing list
> >> RASMB at rasmb.bbri.org
> >> http://rasmb.bbri.org/cgi-bin/mailman/listinfo/rasmb
> >>
> >
> >
> >
> > _______________________________________________
> > RASMB mailing list
> > RASMB at rasmb.bbri.org
> > http://rasmb.bbri.org/cgi-bin/mailman/listinfo/rasmb
>
> --
> Dr Andrew Leech * Laboratory Head
> Technology Facility * Molecular Interactions Laboratory
> Department of Biology (Area 15) * Tel : +44 (0)1904 328723
> University of York * Fax : +44 (0)1904 328804
> PO Box 373, York YO10 5YW * Email : apl3 at york.ac.uk
> EMAIL DISCLAIMER: http://www.york.ac.uk/docs/disclaimer/email.htm
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--
Virgil Schirf
Technical Director, Center for Macromolecular Interactions
<http://cmmi.uthscsa.edu>
Department of Biochemistry, Rm 4.523U
The University of Texas Health Science Center
7703 Floyd Curl Dr.
San Antonio TX 78229-3900
Voice mail: 210 567-6590
Fax: 210 567-6595
Email: schirfv at biochem.uthscsa.edu <mailto:schirfv at biochem.uthscsa.edu>
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