[RASMB] determination of phage particle concentration

[Carlos E. Catalano]

sabine,
we have used the EM method to quantify total number of particles using  
polystyrene beads as an internal standard.  you must titer to quantify  
infective particles, of course.  uv method will work fine, but assumes  
that all of the absorbing species are actually proteins incorporated  
into a viral particle (i.e., will also detect partially-assembled  
structures).
peter - the nanosight sounds interesting ...
cec
=========================================
Carlos Enrique Catalano, Pharm.D., Ph.D.
Professor of Medicinal Chemistry
University of Washington School of Pharmacy
172H Health Science Building
Box 357610
Seattle, WA  98195-7610
(206) 685-2468  (office)
(206) 685-3252  (fax)
catalanc at u.washington.edu
http://depts.washington.edu/medchem/faculty/Catalano.html






On Mar 4, 2010, at 2:57 AM, kaltofen at uni-potsdam.de wrote:

Dear all,

we are trying to obtain the concentration of bacteriophages in a  
solution, concentration meaning the number of particles per ml. The  
solution is supposed to be monodisperse and the MW is (roughly) known  
but could be determined exactly. Is the any idea of how to do this  
with a hydrodynamic method (staining is difficult to compare between  
phage mutants and counting infected cells is tedious). I`ve got the  
"feeling" that one could use the number-averaged MW, but maybe this is  
totally wrong.

Thank you for any hints, literature is also welcome.

Cheers,

Sabine




Sabine Kaltofen
PhD student

Universität Potsdam
Department of Physical Biochemistry
Institute of Biochemistry and Biology
Karl-Liebknecht-Str. 24-25, Haus 25, Raum B/0.05
D-14476 Potsdam-Golm
Telefon: +49-(331)-977-5245
Email: kaltofen at uni-potsdam.de



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