[RASMB] Xl-I wavelength check question

[Karel Planken]

Dear Mark,
 
Just measure through a windowless cell (complete cell without windows). The weight difference with the counterbalance may be up to 5 g but as long as the rotor is not spinning above 3000 rpm you're safe. You may want to look up the appendix in the hardware manual available at "http://ultrascan.uthscsa.edu/aucmanual/AUCuserGuideVolume-1-Hardware.pdf <http://ultrascan.uthscsa.edu/aucmanual/AUCuserGuideVolume-1-Hardware.pdf> ". Good luck,
 
Karel Planken
 
Dr. K.L. Planken
 
Max-Planck-Institute of Colloids and Interfaces
Colloid Chemistry
 
Am Mühlenberg 1
D-14476 Golm
Germany
 
Tel.: +49 331 567 9518
Fax:  +49 331 567 9502
Office:K-2.102
E-mail: karel.planken at mpikg.mpg.de.

________________________________

From: rasmb-bounces at rasmb.bbri.org [mailto:rasmb-bounces at rasmb.bbri.org] On Behalf Of Mark Agacan
Sent: Wednesday, 17. February 2010 11:49
To: rasmb at server1.bbri.org
Subject: [RASMB] Xl-I wavelength check question


I have a question regarding the wavelength accuracy check for the XL-I:
 
Using a windowless cell (aluminium cell housing only with no centerpiece or windows, or one of the empty holes in the rotor?) - how can I match the weight of this cell with the counterbalance, even at the relatively low speed of 3000 rpm?

The remainder of the procedure as outlined in the Troubleshooting and Maintenance section of the XL-I manual seems straighforward enough, i'm just a little unclear on exactly how to load the rotor for this measurement.
 
Best Regards
 
Mark
 

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Mark Agacan, Scientific Officer for the Division of Biological Chemistry and Drug Discovery,
Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dundee, DD1 5EH 
Tel: +44 1382 386095 Fax: +44 1382 345764 Mobile: 07525 451 117
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